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The extracellular matrix in development and disease deals with the molecular and cellular aspects of development and disease. Cells exist in three-dimensional scaffolding called the extracellular matrix. The matrix holds together the millions of cells that make up our blood vessels, organs, skin, and all tissues of the body. The matrix serves as a reservoir of signaling molecules as well. In bacterial cultures, biofilms form as an extracellular matrix and play essential roles in disease and drug resistance. Topics such as matrix structure and function, cell attachment and cell surface proteins mediating cell-matrix interactions, synthesis, regulation, composition, structure, assembly, remodeling, and function of the matrix are included. A common thread uniting the topics is the essential nature that the matrix plays in normal development and pathophysiology. Providing new knowledge will lead us to improved diagnostics, the preventions of disease progression, and therapeutic strategies for the repair and regeneration of tissues. Topics such as the extracellular matrix in hereditary diseases, reproduction, cancer, muscle, and tissue engineering applications, and diverse roles for integrins, are included in this collection.

tissue engineering --- embryonic development --- biofilm --- collagen --- fibrosis --- extracellular matrix --- degenerative disease

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This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development. Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Embryology. --- Laboratory animals. --- Animal genetics. --- Animal Models. --- Animal Genetics and Genomics. --- Genetics --- Animals, Experimental --- Animals, Laboratory --- Animals in research --- Experimental animals --- Lab animals --- Animal culture --- Laboratory organisms --- Working animals --- Animal experimentation --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Embryology --- Development

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This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics. Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation.

Embryology. --- Cell culture. --- Laboratory animals. --- Cell Culture. --- Animal Models. --- Animals, Experimental --- Animals, Laboratory --- Animals in research --- Experimental animals --- Lab animals --- Animal culture --- Laboratory organisms --- Working animals --- Animal experimentation --- Cultures (Biology) --- Cytology --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Technique --- Embryology --- Development

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"Every student will be affected by pregnancy, either their mother's, because what happens in the womb does not necessarily stay in the womb, or by someone else's. As health care professionals, you will often encounter women of childbearing age who may be pregnant, or you may have children of your own, or maybe it is a friend who is pregnant. In any case, pregnancy and childbirth are relevant to all of us, and unfortunately, these processes often culminate in negative outcomes. For example, 50% of all embryos are spontaneously aborted. Furthermore, prematurity and birth defects are the leading causes of infant mortality and major contributors to disabilities. Fortunately, new strategies can improve pregnancy outcomes, and health care professionals have a major role to play in implementing these initiatives. However, a basic knowledge of embryology is essential to the success of these strategies, and with this knowledge, every health care professional can play a role in providing healthier babies. To accomplish its goal of providing a basic understanding of embryology and its clinical relevance, Langman's Medical Embryology retains its unique approach of combining an economy of text with excellent diagrams and clinical images. It stresses the clinical importance of the subject by providing numerous clinical examples that result from abnormal embryological events. The following pedagogic features and updates in the 14th edition help facilitate student learning"--Provided by publisher.

Fetus --- Embryology --- Abnormalities, Human --- Embryonic Development --- Fetal Development --- Congenital Abnormalities --- Abnormalities --- Anomalies, Congenital --- Birth defects --- Congenital abnormalities --- Congenital anomalies --- Defects, Birth --- Deformities --- Developmental abnormalities --- Human abnormalities --- Malformations, Congenital --- Morphology --- Pathology --- Teratogenesis --- Teratology --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Fetal development --- Intrauterine development --- Abnormalities, Congenital --- Defects, Congenital --- Birth Defects --- Congenital Defects --- Abnormality, Congenital --- Birth Defect --- Congenital Abnormality --- Congenital Defect --- Defect, Birth --- Defect, Congenital --- Deformity --- Fetal Diseases --- Infant, Newborn, Diseases --- Fetal Growth --- Fetal Programming --- Development, Fetal --- Fetal Programmings --- Growth, Fetal --- Embryonic Programming --- Post-implantation Embryo Development --- Postnidation Embryo Development --- Postnidation Embryo Development, Animal --- Pre-implantation Embryo Development --- Prenidation Embryo Development, Animal --- Embryo Development --- Postimplantation Embryo Development --- Preimplantation Embryo Development --- Development, Embryo --- Development, Postnidation Embryo --- Embryo Development, Post-implantation --- Embryo Development, Postimplantation --- Embryo Development, Postnidation --- Embryo Development, Pre-implantation --- Embryo Development, Preimplantation --- Embryonic Developments --- Embryonic Programmings --- Post implantation Embryo Development --- Pre implantation Embryo Development --- Embryo, Mammalian --- Embryo, Nonmammalian --- Development --- growth & development --- Embryologie humaine --- Foetus --- Malformations --- Embryology, Human. --- Abnormalities, Human. --- Embryonic Development. --- Fetal Development. --- Congenital Abnormalities. --- Development. --- Human embryology --- Fetal Anomalies --- Fetal Malformations --- Anomaly, Fetal --- Fetal Anomaly --- Fetal Malformation --- Malformation, Fetal --- Malformations foetales. --- Embryologie humaine.