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This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.

Plant genetics. --- Plant Genetics and Genomics. --- Plants --- Genetics --- Genòmica --- Genètica vegetal --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Millorament selectiu de plantes --- Genètica molecular --- Metagenòmica --- Nutrigenòmica --- Genomes

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This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.

Radiology, Medical. --- Imaging / Radiology. --- Clinical radiology --- Radiology, Medical --- Radiology (Medicine) --- Medical physics --- Gens --- Genètica molecular --- Radiologia mèdica --- Radiologia clínica --- Radiologia (Medicina) --- Física mèdica --- Medicina nuclear --- Radiofàrmacs --- Radiografia mèdica --- Radiologia dental --- Radiologia intervencionista --- Radiologia pediàtrica --- Radiologia veterinària --- Radioteràpia --- Diagnòstic radiològic --- Biologia molecular --- Genètica --- Clonatge molecular --- Codi genètic --- Elements genètics mòbils --- Genètica bioquímica --- Genètica molecular humana --- Genètica molecular vegetal --- Genòmica --- Regulació genètica --- Variació en el nombre de còpies --- Unitats d'informació genètica --- Herència (Biologia) --- ADN --- Medical radiology.