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This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field.
Botany. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Floristic botany --- Cèl·lules eucariotes --- Biologia molecular vegetal --- Manuals de laboratori --- Tècniques de laboratori --- Biologia molecular de les plantes --- Biologia molecular --- Genètica molecular vegetal --- Fisiologia vegetal --- Fitoquímica --- Protists --- Cèl·lules --- Cèl·lules animals
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This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various techniques to analyze plastids through means of combining biology strategies from genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field.
Botany. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Floristic botany --- Cèl·lules eucariotes --- Cèl·lules i teixits vegetals --- Biologia molecular vegetal --- Manuals de laboratori --- Tècniques de laboratori --- Biologia molecular de les plantes --- Biologia molecular --- Genètica molecular vegetal --- Fisiologia vegetal --- Fitoquímica --- Cèl·lules vegetals --- Histologia vegetal --- Teixits vegetals --- Anatomia vegetal --- Cèl·lules --- Teixits (Histologia) --- Espores de plantes --- Parets cel·lulars vegetals --- Sistema vascular de les plantes --- Xilema --- Protists --- Cèl·lules animals
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