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Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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This edited work presents useful methods in experimenting in the area of Bioprocessing and Biotechnology. The four sections cover the area of Bioprocess, Whole Cells & Isolated Biocatalyst, Characterization of Biochemical Products and Cell Isolation & Culture. Its enable researchers to choose a suitable method and plan their experiments in details. The main focus of this book is to provide step by step method to young researchers, especially in the new research areas. Among the latest areas are the isolation of novel strain or enzyme by metagenomic approaches and taming procedure in the laboratories, development of novel, the cheap and non-toxic catalyst for biodiesel production, and production of micro-fibrillated cellulose. An updated method for well-known areas such as immobilization technology, biosensor, and polymerization was also presented. The book also covers in-silico methods such as MATLAB platform to ease researchers. Not to forget, the method in animal and plant culture is also discussed in detail. The book is written by chapter authors with much expertise in their fields. They have published multiple articles in the index listed journals. The topic of this book is particularly relevant to young researchers who are struggling to fine-tune their research and do not want to waste their time in optimizing the experiment set up.

Biotechnology. --- Biochemistry. --- Protein Science. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Molecule Analysis: Methods and Protocols, Second Edition aims to be useful to many researchers, inspire them and help them to go single molecule. .

Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response.  Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently workingin the B cell field.

Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition