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Biology

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Biology.

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Es wird die Entwicklung eines modularen Robotersystems beschrieben, das bisher manuell erledigte Arbeitsschritte automatische durchführt. Ziel einer solchen Automatisierung ist einerseits den nötigen Persomal- und Kostenaufwand zu reduzieren und wissenschaftiche Erkentnisse schneller und leichter zu erzielen. Hierzu werden ein Roboter zur Probevorbereitung und ein Roboter zur zweistufigen Untersuchung von Proben mit automatischer Erkennung des interessierenden Bereiches entwickelt. Ein weiterer Aspekt ist die Steigerung der Effizienz hochauflösender, bildgebender Laboranalytik. Hierzu werden zwei einfache automatische bildgebende Roboter entwickelt, mit denen eine Vorauswahl der für weitere Untersuchungen interessanten Proben automatisch durchgeführt wird. Um eine automatische Gesamtprozesskette zu ermöglichen, wird ein auf die Einzelroboter abgestimmtes Transportsystem entwickelt, das den Transport von Mikrotiterplatten zwischen den Funktionseinheiten übernimmt. Zur Umsetzung der Einzelfunktionen werden Bildverarbeitungsmittel zur Erkennung von Informationen in Bildern in automatischen Algorithmen implementiert. High-throughput testing is gaining importance today for managing and analyzing imaging procedures in industry and research, especially in the medical technology and pharmaceutical industries and in the life sciences. This book presents a new design for an automatic process chain that is based on a modular robotic system, enabling the efficient management of large quantities of data.

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This volume uniquely engages biology students in active learning, scientific thinking and skill development. It is beloved for its Socratic narrative style, its emphasis on experimental evidence and its dedication to active learning.

Biology. --- Biology --- Science. --- Science.

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The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists