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This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.

Computational Intelligence. --- Pharmaceutical Sciences/Technology. --- Computational Biology/Bioinformatics. --- Engineering, Protein --- Protein design --- Engineering. --- Biotechnology. --- Pharmaceutical technology. --- Bioinformatics. --- Biomedical engineering. --- Biomedical Engineering. --- Protein engineering. --- Proteins --- Biochemical engineering --- Genetic engineering --- Design --- Biomedical Engineering and Bioengineering. --- Pharmaceutical laboratory techniques --- Pharmaceutical laboratory technology --- Technology, Pharmaceutical --- Technology --- Chemical engineering --- Construction --- Industrial arts --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Data processing --- Computational intelligence. --- Intelligence, Computational --- Artificial intelligence --- Soft computing