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Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.

General biochemistry --- Molecular biology --- Immunology. Immunopathology --- Physiology of nerves and sense organs --- Pathological biochemistry --- Human medicine --- monoklonale antilichamen --- polyklonale antilichamen --- immunologie --- medische biochemie --- neurochemie --- farmacologie --- stamcellen --- biochemie --- biomedische wetenschappen --- moleculaire biologie

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The present book addresses the multi-disciplinary nature of Translational Outcomes Research, which is a watershed for nearly all the disciplines of Life and Health Sciences, along with the Materials Sciences including but not limited to Zoology, Botany, Microbiology, Biochemistry, Physiology, Nanotechnology, the Medical Sciences, Bioengineering, Biophysics, Medicinal Chemistry, Structural Biology, Biostatistics and Bioinformatics. This book, for the first time, addresses the basic premises of fundamental research in facilitating drug discovery. One chapter is dedicated to a novel generation of platforms with novel camelid antibodies and their technological extensions, while another focuses on functional food and nutraceuticals. The book begins with a thorough overview of what translational outcomes research connotes and what the current status of research in the area is, and goes on to elucidate various pertinent preclinical disease models and their uses in basic and application based research in the Life Sciences. How basic approaches to screening and characterization vis-à-vis their role in amelioration of the two cardinal problems of inflammation and degeneration involved in most diseases is elucidated. The book ends with a discussion of the relevance and importance of using Bio Green technology in Translational Outcomes, addressing the need to fill the gap between academia and industry and clinics that can arise through direct or indirect collaboration between the stakeholders and emphasizing the need for an eco-friendly approach so as not to jeopardize the fine balance that holds life on earth in harmony.

Science --- Molecular biology --- Biology --- Animal physiology. Animal biophysics --- Immunology. Immunopathology --- Pharmacology. Therapy --- Human medicine --- Manufacturing technologies --- monoklonale antilichamen --- polyklonale antilichamen --- immunologie --- mens-dier vergelijkend onderzoek --- bio-informatica --- farmacologie --- groene chemie --- biologie --- geneeskunde --- gentherapie --- histologie --- moleculaire biologie --- proefdieren