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This third edition volume expands on the previous editions both by presenting more detailed protocols for the techniques described in the first and second editions of High Throughput Screening: Methods and Protocols and by covering important new procedures. The first chapter of this book provides an overview of important assay development techniques, while the rest of the chapters detail how to develop and execute screens at whatever throughput the user needs. Some chapter examples are: structure-based virtual screening, high throughput screening using mass spectrometry, identification of state-dependent blockers for voltage gated calcium channels, bioluminescence resonance energy transfer platform to monitor protein-protein interactions in live cells, high throughput flow cytometry, and application of imaging-based assays in microplate formats for high content screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, High Throughput Screening: Methods and Protocols, Third Edition, is a valuable resource for anyone who is interested in HTS research.
Biotechnology. --- Chemical engineering --- Genetic engineering
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Biotechnology. --- Chemical engineering --- Genetic engineering
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Biotechnology. --- Biotechnology --- Chemical engineering --- Genetic engineering
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Social movements --- Environmental policy --- Plant genetic engineering.
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Crops --- Plant biotechnology --- Agricultural biotechnology --- Genetic engineering
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In 'Soybeans and Power,' Pablo Lapegna investigates the ways in which rural populations cope with GM soybean expansion in Argentina, a major player in the use and export of GM crops. Based on over a decade of ethnographic research, Lapegna reveals the reasons why many local communities initially resisted, yet ultimately accepted GM crops.
Social movements --- Environmental policy --- Plant genetic engineering.
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Rapid developments in the manipulation of genomes, including editing genes with 'molecular scissors' and the synthesising of new lifeforms look set to transform our future, and perhaps that of life on Earth. John Parrington explains the cutting edge science and its implications.
Genomics. --- Genetic engineering. --- Genomes. --- Génome. --- Génomique. --- Génie génétique. --- Genomics --- Genetic Engineering --- Gene Editing. --- trends.
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Biotechnology, Second Edition approaches modern biotechnology from a molecular basis, which has grown out of increasing biochemical understanding of genetics and physiology. Using straightforward, less-technical jargon, Clark and Pazdernik introduce each chapter with basic concepts that develop into more specific and detailed applications. This up-to-date text covers a wide realm of topics including forensics, bioethics, and nanobiotechnology using colorful illustrations and concise applications. In addition, the book integrates recent, relevant primary research articles for each chapter, which are presented on an accompanying website. The articles demonstrate key concepts or applications of the concepts presented in the chapter, which allows the reader to see how the foundational knowledge in this textbook bridges into primary research. This book helps readers understand what molecular biotechnology actually is as a scientific discipline, how research in this area is conducted, and how this technology may impact the future.
Biotechnology --- Genetic engineering --- Molecular biology --- Biotechnologie --- 57.08 --- Biological techniques --- Biotechnologie. --- Génie génétique. --- Biologie moléculaire. --- Genetic Engineering --- Molecular Biology --- Biotechnology - Textbooks --- Genetic engineering - Textbooks --- Molecular biology - Textbooks
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biotechnologie --- moleculaire biologie --- Molecular biology --- Biotechnology. --- Genetic Engineering. --- Molecular Biology. --- Genetic engineering. --- Biotechnologie --- Biologie moléculaire --- Génie génétique --- Biotechnology --- Genetic Engineering --- Molecular Biology --- Biotechnologie. --- Biologie moléculaire. --- Génie génétique. --- Chemistry
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In the mid-twentieth century, American plant breeders, frustrated by their dependence on natural variation in creating new crops and flowers, eagerly sought technologies that could extend human control over nature. Their search led them to celebrate a series of strange tools: an x-ray beam directed at dormant seeds, a drop of chromosome-altering colchicine on a flower bud, and a piece of radioactive cobalt in a field of growing crops. According to scientific and popular reports of the time, these mutation-inducing methods would generate variation on demand, in turn allowing breeders to genetically engineer crops and flowers to order. Creating a new crop or flower would soon be as straightforward as innovating any other modern industrial product. In Evolution Made to Order, Helen Anne Curry traces the history of America's pursuit of tools that could speed up evolution. It is an immersive journey through the scientific and social worlds of midcentury genetics and plant breeding and a compelling exploration of American cultures of innovation. As Curry reveals, the creation of genetic technologies was deeply entangled with other areas of technological innovation-from electromechanical to chemical to nuclear. An important study of biological research and innovation in America, Evolution Made to Order provides vital historical context for current worldwide ethical and policy debates over genetic engineering.
Plant mutation breeding --- Plant genetic engineering --- History --- Social aspects --- Genetic engineering --- agricultural biotechnology. --- biotechnology. --- evolution. --- genetic engineering. --- genetic technologies. --- mutation breeding. --- plant breeding. --- technological innovation.
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