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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers r

Esterases --- Computational Biology --- DNA Restriction-Modification Enzymes --- Endodeoxyribonucleases --- Genetic Techniques --- Genetics --- Deoxyribonucleases --- Enzymes --- Hydrolases --- Biology --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biological Science Disciplines --- Enzymes and Coenzymes --- Natural Science Disciplines --- Chemicals and Drugs --- Disciplines and Occupations --- DNA Restriction Enzymes --- Genomics --- Endonucleases --- Genetic Engineering --- Health & Biological Sciences --- Genomics. --- Genome research --- Genomes --- Research --- Molecular genetics

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This volume focuses on recent advances in the biochemical and molecular analysis of different families of phospholipases in plants and their roles in signaling plant growth, development, and responses to abiotic and biotic cues.   The hydrolysis of membrane lipids by phospholipases produces different classes of lipid mediators, including phosphatidic acid, diacylglycerol, lysophospholipids, free fatty acids, and oxylipins.  Phospholipases are grouped into different families and subfamilies according to their site of hydrolysis, substrate usage, and sequence similarities.  Activating one or more of these enzymes often constitutes an early, critical step in many regulatory processes, such as signal transduction, vesicular trafficking, secretion, and cytoskeletal rearrangements.  Lipid-based signaling plays pivotal roles in plant stress responses, cell size, shape, growth, apoptosis, proliferation, and reproduction.

Lipids -- Physiology. --- Plant lipids. --- Plants -- Chemistry. --- Human Anatomy & Physiology --- Botany --- Health & Biological Sciences --- Earth & Environmental Sciences --- Plant Physiology --- Animal Biochemistry --- Phospholipases. --- Plant cell interaction. --- Life sciences. --- Plant biochemistry. --- Lipids. --- Plant pathology. --- Plant physiology. --- Life Sciences. --- Plant Physiology. --- Plant Biochemistry. --- Lipidology. --- Plant Pathology. --- Cell interaction --- Plant cellular control mechanisms --- Esterases --- Biochemistry. --- Plant diseases. --- Communicable diseases in plants --- Crop diseases --- Crops --- Diseases of plants --- Microbial diseases in plants --- Pathological botany --- Pathology, Vegetable --- Phytopathology --- Plant pathology --- Plants --- Vegetable pathology --- Agricultural pests --- Crop losses --- Diseased plants --- Phytopathogenic microorganisms --- Plant pathologists --- Plant quarantine --- Lipides --- Lipins --- Lipoids --- Biomolecules --- Steroids --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Physiology --- Pathology --- Diseases and pests --- Diseases --- Wounds and injuries --- Composition --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics --- Plant cellular signal transduction.