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Enzymes --- Industrial enzymology --- Industrial applications.

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In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought together a number of core protocols concentrating on Protein

Clinical enzymology. --- Enzymes. --- Immobilized enzymes -- Laboratory manuals. --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Immobilized enzymes --- Insoluble enzymes --- Localized enzymes --- Water-insoluble enzymes --- Biocatalysts --- Ferments --- Soluble ferments --- Diagnostic enzymology --- Enzymes --- Immobilized proteins --- Catalysts --- Proteins --- Enzymology --- Clinical chemistry

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers r

Esterases --- Computational Biology --- DNA Restriction-Modification Enzymes --- Endodeoxyribonucleases --- Genetic Techniques --- Genetics --- Deoxyribonucleases --- Enzymes --- Hydrolases --- Biology --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biological Science Disciplines --- Enzymes and Coenzymes --- Natural Science Disciplines --- Chemicals and Drugs --- Disciplines and Occupations --- DNA Restriction Enzymes --- Genomics --- Endonucleases --- Genetic Engineering --- Health & Biological Sciences --- Genomics. --- Genome research --- Genomes --- Research --- Molecular genetics

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"Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Offers project lab formats for students that closely simulate original research projects. Provides instructional guidance for students to design their own experiments . Includes advanced analytical techniques. Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH. Includes access to a website with additional resources for instructors"--Page 4 of cover.

Enzymes --- Publication Formats --- Enzymes and Coenzymes --- Chemicals and Drugs --- Publication Characteristics --- Laboratory Manuals --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Analysis --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology

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Volume 542 of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This new volume covers research methods providing a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as ""yet another"" general hallmark of ca

Clinical enzymology. --- Enzymes. --- Oncometabolism. --- Diseases --- Metabolism --- Metabolic Phenomena --- Phenomena and Processes --- Neoplasms --- Energy Metabolism --- Medicine --- Health & Biological Sciences --- Pathology --- Diagnostic enzymology --- Clinical chemistry --- Enzymology