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This thesis, authored by Alina Sekretaryova, focuses on the development of novel reagentless electrodes for biosensing applications. It addresses challenges in analytical chemistry, particularly the need for more accurate and sensitive biosensors for real-time analysis in fields such as healthcare, pharmacology, and environmental chemistry. The work emphasizes the use of immobilized oxidases and phenothiazine as a hydrophobic mediator to enhance the stability and performance of biosensors. Sekretaryova explores the production of screen-printed graphite microband electrodes to improve sensitivity and enable mass production. The thesis also introduces a self-powered biosensor design, capable of generating an electrical current upon analyte detection, such as cholesterol in plasma. The research is geared towards scientific and industrial audiences interested in biosensor technology and its potential applications.

Biosensors. --- Electrodes. --- Biosensors --- Electrodes

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Based on the mature development of microfabrication technology, micro-scale electrodes, i.e., microelectrodes, for the electrical observation of activities in micro-scale environment become realizable. This technique opened a new avenue for micro-scale observation and measurement besides the optical technique. This book presents the techniques, structures, and applications of using microelectrodes in a micro-scale environment. It covers a broad spectrum of topics and the state of the art techniques in the field. As such, the book is divided into four sections, comprising 11 chapters written by

Microelectrodes. --- Biosensors. --- Biodetectors --- Biological detectors --- Biological sensors --- Biomedical detectors --- Biomedical sensors --- Detectors --- Medical instruments and apparatus --- Physiological apparatus --- Miniature electrodes --- Electrodes

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Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.

Immunology. --- Cytokines. --- Cytokines and Growth Factors. --- Cellular immunity --- Immune response --- Immunobiology --- Life sciences --- Serology --- Regulation --- Biological Assay. --- Biosensing Techniques. --- Biosensing Technics --- Bioprobes --- Biosensors --- Electrodes, Enzyme --- Bioprobe --- Biosensing Technic --- Biosensing Technique --- Biosensor --- Electrode, Enzyme --- Enzyme Electrode --- Enzyme Electrodes --- Technic, Biosensing --- Technics, Biosensing --- Technique, Biosensing --- Techniques, Biosensing --- Wearable Electronic Devices --- Microchemistry --- Assay, Biological --- Assays, Biological --- Biologic Assay --- Biologic Assays --- Bioassay --- Assay, Biologic --- Assays, Biologic --- Bioassays --- Biological Assays --- Cytokine

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Frieder W. Scheller, Aysu Yarman, Till Bachmann, Thomas Hirsch, Stefan Kubick, Reinhard Renneberg, Soeren Schumacher, Ulla Wollenberger, Carsten Teller and Frank F. Bier Future of Biosensors: A Personal View Yeon Seok Kim and Man Bock Gu Advances in Aptamer Screening and Small Molecule Aptasensors Pui Sai Lau and Yingfu Li Exploration of Structure-Switching in the Design of Aptamer Biosensors Yu Xiang, Peiwen Wu, Li Huey Tan and Yi Lu DNAzyme-Functionalized Gold Nanoparticles for Biosensing Maren Lönne, Guohong Zhu, Frank Stahl and Johanna-Gabriela Walter Aptamer-Modified Nanoparticles as Biosensors Mahmoud Labib and Maxim V. Berezovski Electrochemical Aptasensors  for Microbial and Viral Pathogens Koichi Abe, Wataru Yoshida and Kazunori Ikebukuro Electrochemical Biosensors Using Aptamers for Theranostics Butaek Lim and Young-Pil Kim Enzymatic Glucose Biosensors Based on Nanomaterials Yong Duk Han, Yo Han Jang and Hyun C. Yoon Cascadic Multienzyme Reaction-Based Electrochemical Biosensors Sven C. Feifel, Andreas Kapp and Fred Lisdat Protein Multilayer Architectures on Electrodes for Analyte Detection Fabiana Arduini and Aziz Amine Biosensors Based on Enzyme Inhibition.

Chemistry. --- Analytical biochemistry. --- Biochemical engineering. --- Biochemistry. --- Biochemical Engineering. --- Biochemistry, general. --- Electrochemistry. --- Analytical Chemistry. --- Biosensors --- Enzymes --- Metabolic Phenomena --- Oligonucleotides --- Molecular Probe Techniques --- Enzymes and Coenzymes --- Investigative Techniques --- Polynucleotides --- Chemicals and Drugs --- Phenomena and Processes --- Nucleotides --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Nucleic Acids, Nucleotides, and Nucleosides --- Aptamers, Nucleotide --- Biosensing Techniques --- Metabolism --- Health & Biological Sciences --- Biomedical Engineering --- Biotechnology --- Biocatalysts --- Metabolic Phenomenon --- Metabolic Process --- Metabolism Concepts --- Metabolism Phenomena --- Process, Metabolic --- Processes, Metabolic --- Anabolism --- Catabolism --- Metabolic Concepts --- Metabolic Processes --- Concept, Metabolic --- Concept, Metabolism --- Concepts, Metabolic --- Concepts, Metabolism --- Metabolic Concept --- Metabolism Concept --- Phenomena, Metabolic --- Phenomena, Metabolism --- Phenomenon, Metabolic --- Biosensing Technics --- Bioprobes --- Electrodes, Enzyme --- Bioprobe --- Biosensing Technic --- Biosensing Technique --- Biosensor --- Electrode, Enzyme --- Enzyme Electrode --- Enzyme Electrodes --- Technic, Biosensing --- Technics, Biosensing --- Technique, Biosensing --- Techniques, Biosensing --- Nucleotide Aptamers --- Oligonucleotide Ligands, DNA --- Oligonucleotide Ligands, RNA --- DNA Aptamers --- RNA Aptamers --- Aptamers, DNA --- Aptamers, RNA --- DNA Oligonucleotide Ligands --- RNA Oligonucleotide Ligands --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Coenzymes and Enzymes --- Molecular Probe Technic --- Molecular Probe Technics --- Molecular Probe Technique --- Technic, Molecular Probe --- Technics, Molecular Probe --- Technique, Molecular Probe --- Techniques, Molecular Probe --- Probe Technic, Molecular --- Probe Technics, Molecular --- Probe Technique, Molecular --- Probe Techniques, Molecular --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Bio-process engineering --- Bioprocess engineering --- Analytic biochemistry --- Ferments --- Soluble ferments --- Biodetectors --- Biological detectors --- Biological sensors --- Biomedical detectors --- Biomedical sensors --- Composition --- Analytical chemistry. --- Biosensors. --- Biotechnology. --- Detectors --- Medical instruments and apparatus --- Physiological apparatus --- Biochemistry --- Chemistry, Analytic --- Physical sciences --- Biology --- Chemistry --- Medical sciences --- Chemical engineering --- Analysis, Chemical --- Analytic chemistry --- Chemical analysis --- Chemistry, Physical and theoretical --- Analytical biochemistry --- Biochemical engineering