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Microscopy --- Ultrastructure (Biology) --- Microscopy. --- Fine structure (Biology) --- Nanobiology --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Cells --- Microstructure --- Nanoscience --- Optics
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For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology. Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales. Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution. The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography; and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging. Written in the successful format of the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.
Radiology, Medical. --- Microscopy. --- Imaging / Radiology. --- Biological Microscopy. --- Clinical radiology --- Radiology, Medical --- Radiology (Medicine) --- Medical physics --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics
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The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment. Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods. Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed descriptions and implementation advice necessary to ensure successful results. Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science.
Biochemistry. --- Microscopy. --- Protein Science. --- Biological Microscopy. --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.
Radiology, Medical. --- Microscopy. --- Imaging / Radiology. --- Biological Microscopy. --- Clinical radiology --- Radiology, Medical --- Radiology (Medicine) --- Medical physics --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics
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The development of high-quality foods with desirable properties for both consumers and the food industry requires a comprehensive understanding of food systems and the control and rational design of food microstructures. Food microstructures reviews best practice and new developments in the determination of food microstructure.After a general introduction, chapters in part one review the principles and applications of various spectroscopy, tomography and microscopy techniques for revealing food microstructure, including nuclear magnetic resonance (NMR) methods, environmental scanning e
Food -- Analysis. --- Food. --- Food --- Microstructure --- Health & Biological Sciences --- Diet & Clinical Nutrition --- Composition --- Analysis --- Microscopy. --- Analysis. --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Analysis of food --- Chemistry of food --- Food, Chemistry of --- Food chemistry --- Chemistry, Technical --- Sanitary chemistry --- Chemistry --- Microstructure. --- Composition.
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The Ultrastructure of Human Tumours: Applications in Diagnosis and Research describes the core features as seen by transmission electron microscopy, defining the different types of cellular differentiation in tumours; this is relevant for tumour nomenclature and diagnosis, which, in turn, are important for tumour pathologists in their collaboration with oncologists for the treatment of cancer patients. The book is divided into 8 chapters. Following an introduction on technique and procedure, there are chapters on epithelial tumours, melanocytic lesions, soft-tissue and related tumours, lymphoma and leukemia, CNS neoplasms and neuroendocrine and neuronal tumours. Each chapter includes an introductory text that puts the ultrastructural features in the context of classical pathology. The book includes many new findings and interpretations from well-known tumours, as well as ultrastructural information on several newly described tumour entities not dealt with in existing tumour ultrastructure monographs. The book will especially be of value to tumour pathologists who need to solve problem cases with the aid of electron microscopy, but also to cancer research and tissue engineering scientists working to develop anti-cancer and stem-cell-based therapies. However, even those without access to electron microscopy may also benefit from this book, since many of the images provide an ‘explanation’ of the appearances of cells, tissues and tumours familiar to pathologists and scientists from light microscopy. In this respect, it is hoped that this book will stimulate the wider use of electron microscopy in pathology. The book is comprehensively referenced, 680 pages long and lavishly illustrated with 757 figures. Dr. Brian Eyden is Consultant Clinical Scientist and Dr. S. Sankar Banerjee Consultant Histopathologist in the Department of Histopathology, Christie NHS Foundation Trust, Manchester, UK; Dr. Yongxin Ru is Director of the Department of Electron Microscopy, Institute of Hematology and Blood Diseases Hospital, Tianjin, China; Paweł Liberski is Professor in the Department of Molecular Pathology and Neuropathology, Medical University Łódź, Poland.
Medicine. --- Microscopy. --- Oncology. --- Pathology. --- Tumors. --- Ultrastructure (Biology). --- Medicine --- Health & Biological Sciences --- Oncology --- Ultrastructure (Biology) --- Fine structure (Biology) --- Nanobiology --- Neoplasms --- Tumours --- Cancer research. --- Biomedicine. --- Cancer Research. --- Biological Microscopy. --- Pathology --- Cysts (Pathology) --- Cells --- Microstructure --- Nanoscience --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine, Preventive --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Tumors --- Cancer research
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Highlighting recent advances in our understanding of breast cancer, this book is intended for a wide audience as a reference book. Included are reviews of genetics, epigenetics, various aspects of cell and molecular biology, and several other areas of breast cancer that are aimed at determining new intervention sites for treatments and cures of the disease. The chapters are written by internationally recognized experts and include reviews of key topics in breast cancer research. Each chapter highlights the new aspects of specific research topics, the various impacts of designing new strategies, and identifies new targets for therapeutic intervention. The topics addressed are selected to be of interest to patients, scientists, students, teachers, and to all who are interested in expanding their knowledge of breast cancer imaging, diagnostics, therapeutics, or basic biomedical research on breast cancer.
Communicable diseases -- Epidemiology. --- Epidemiology. --- Molecular biology. --- Breast --- Neoplasms by Site --- Biology --- Neoplasms --- Biological Science Disciplines --- Diseases --- Natural Science Disciplines --- Disciplines and Occupations --- Breast Neoplasms --- Genetics --- Health & Biological Sciences --- Cytology --- Genetic aspects --- Cancer --- Treatment --- Oncology. --- Cytology. --- Microscopy. --- Cancer. --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Cell biology --- Cellular biology --- Life sciences. --- Cancer research. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Biological Microscopy. --- Cancer Research. --- Optics --- Cells --- Cytologists --- Tumors --- Cancer research
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S. Glöggler B. Blümich S. Appelt NMR Spectroscopy for Chemical Analysis at Low Magnetic Fields U.L. Günther Dynamic Nuclear Hyperpolarization in Liquids E. Kupce NMR with Multiple Receivers Y. Xu S. Matthews TROSY NMR Spectroscopy of Large Soluble Proteins H. Müller M. Etzkorn H. Heise Solid-State NMR Spectroscopy of Proteins G. Pintacuda G. Kervern Paramagnetic Solid-State Magic-Angle Spinning NMR Spectroscopy .
Chemistry. --- Spectroscopy. --- Biochemistry. --- Spectroscopy/Spectrometry. --- Protein Structure. --- Spectroscopy and Microscopy. --- Chemistry --- Physical Sciences & Mathematics --- Analytical Chemistry --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Analysis, Spectrum --- Spectra --- Spectrochemical analysis --- Spectrochemistry --- Spectroscopy --- Composition --- Proteins. --- Microscopy. --- Spectrum analysis. --- Biology --- Medical sciences --- Chemistry, Analytic --- Interferometry --- Optics --- Radiation --- Wave-motion, Theory of --- Absorption spectra --- Light --- Spectroscope --- Qualitative --- Spectrometry --- Proteins . --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Analytical chemistry --- Spectroscopie de la résonance magnétique nucléaire
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Cytopreparation: Principles & Practice by Gary W. Gill fills a long-standing need for an easy-to-use and authoritative manual on the fundamentals of cytopreparation up-to-and- including microscopy, screening, and data analysis. The text describes in phenomenological terms the most common materials and methods of specimen collection through mounting for gyn, non-gyn, and FNA specimens, as well as the underlying mechanistic bases. The author provides his expertise and information that will empower and enable readers to review and improve their laboratories’ cytopreparatory techniques as they apply to the vast majority of specimens. This unique volume provides facts that are not readily available anywhere. Cytopreparation: Principles & Practice is intended for everyone associated with, and involved in, making cytologic preparations that are useful for their intended purpose. It will serve as a valuable reference tool for educators in cytology and histology, cytotechnology and histotechnology students, cytotechnologists, cytopreparatory technicians, cytopathologists, anatomical/clinical pathologists, pathology residents and cytopathology fellows.
Cytology --- Cell physiology. --- Technique. --- Cell function --- Cytodiagnosis. --- Exfoliative cytology. --- Diagnostic specimens --- Medicine. --- Laboratory medicine. --- Pathology. --- Microscopy. --- Medicine & Public Health. --- Laboratory Medicine. --- Biological Microscopy. --- Physiology --- Medical laboratories. --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Diagnosis, Laboratory --- Health facilities --- Laboratories --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Clinical medicine --- Clinical pathology --- Diagnostic laboratory tests --- Laboratory diagnosis --- Laboratory medicine --- Medical laboratory diagnosis --- Diagnosis --- Pathology
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This volume presents, for the very first time, an exhaustive collection of those modern numerical methods specifically tailored for the analysis of Strongly Correlated Systems. Many novel materials, with functional properties emerging from macroscopic quantum behaviors at the frontier of modern research in physics, chemistry and material science, belong to this class of systems. Any technique is presented in great detail by its own inventor or by one of the world-wide recognized main contributors. The exposition has a clear pedagogical cut and fully reports on the most relevant case study where the specific technique showed to be very successful in describing and enlightening the puzzling physics of a particular strongly correlated system. The book is intended for advanced graduate students and post-docs in the field as textbook and/or main reference, but also for other researchers in the field who appreciate consulting a single, but comprehensive, source or wishes to get acquainted, in a as painless as possible way, with the working details of a specific technique.
Condensed matter. --- Numerical methods. --- Superconductivity. --- Physics --- Physical Sciences & Mathematics --- Atomic Physics --- Condensed materials --- Condensed media --- Condensed phase --- Materials, Condensed --- Media, Condensed --- Phase, Condensed --- Physics. --- Solid state physics. --- Spectroscopy. --- Microscopy. --- Solid State Physics. --- Spectroscopy and Microscopy. --- Mathematical Methods in Physics. --- Liquids --- Matter --- Solids --- Electric conductivity --- Critical currents --- Superfluidity --- Mathematical physics. --- Physical mathematics --- Mathematics --- Natural philosophy --- Philosophy, Natural --- Physical sciences --- Dynamics --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Analysis, Spectrum --- Spectra --- Spectrochemical analysis --- Spectrochemistry --- Spectrometry --- Spectroscopy --- Chemistry, Analytic --- Interferometry --- Radiation --- Wave-motion, Theory of --- Absorption spectra --- Light --- Spectroscope --- Qualitative --- Analytical chemistry
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