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Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research.  Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.

Oncology. --- Gene expression. --- Cancer Research. --- Gene Expression. --- Tumors --- Genes --- Genetic regulation --- Expression

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Two decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases. Since then, new technologies have developed and innovative concepts have emerged, which may prove useful in devising therapeutic approaches to neurological diseases. Divided into six convenient sections, Trinucleotide Repeat Protocols, Second Edition covers a wide range of topics such as an overview of trinucleotide repeat diseases, synaptic plasticity, embryonic stem (ES) cell-related protocols with a focus on HD, RNA-related protocols, and analysis of epigenetic modification in fragile X syndrome.  This edition focuses not only on direct analysis of trinucleotide repeat diseases but also on alternative approaches for the analysis of trinucleotide repeat diseases, with the hope that this will result in a better understanding of the mechanisms and future therapeutic prospects for treatment of these diseases. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Trinucleotide Repeat Protocols, Second Edition seeks to serve researchers with its thorough methodologies on this expanding field.

Medicine. --- Gene expression. --- Molecular Medicine. --- Gene Expression. --- Genes --- Genetic regulation --- Health Workforce --- Expression

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As imaging technologies and approaches have evolved, the scope of certain imaging techniques has moved far beyond the production of purely illustrative images or appealing time-lapse movies to providing the scientist with a rich range of ways to measure and quantify the biological process and outcome of gene expression.  In Imaging Gene Expression: Methods and Protocols, expert authors offer up-to-date approaches and protocols that scientists in the field have developed, which would benefit the broader scientific community. Divided in three convenient parts, this detailed book covers the output of a gene, namely the RNA molecules that are transcribed from the gene and the way by which these molecules can be tracked or quantified in fixed or living cells, protocols that focus on the gene, DNA, or chromatin, as well as a variety of ways by which nuclear processes intertwined with gene expression can be followed and quantified in living cells as well as approaches for studying several sub-nuclear structures found in eukaryotic cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and up-to-date, Imaging Gene Expression: Methods and Protocols will serve researchers working toward imaging in the context of complete organisms.

Radiology, Medical. --- Gene expression. --- Imaging / Radiology. --- Gene Expression. --- Clinical radiology --- Radiology, Medical --- Radiology (Medicine) --- Medical physics --- Genes --- Genetic regulation --- Expression

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In this volume of Methods in Molecular Biology™, expert investigators offer comprehensive, complementary, and cutting-edge technologies for studies of gene regulation.  The chapters of Gene Regulation: Methods and Protocols are organized to provide an integrated and a coherent view of control systems and their associated components.  The protocols are broad in their scope.  They include molecular, biochemical, spectroscopic techniques as well as high throughput strategies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines including molecular biology, genomics, biochemistry, biomedicine, nutrition, and agricultural sciences.     FEATURES • Techniques for studying chromatin structural features that influence gene activation • In vivo and in vitro assays • Spectroscopic techniques • Technologies for examining genome-wide association of transcription factors with regulatory regions of genes • Proteomics based on SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture) • Peptide microarray technology for studies of kinases • Schemes for isolation and characterization of relatively large multiprotein complexes • Biochemical and biophysical techniques for examining gene repression • In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes • Nuclear recruitment assay to validate transcription factor interactions in mammalian cells.

Human genetics. --- Gene expression. --- Human Genetics. --- Gene Expression. --- Genes --- Genetic regulation --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Expression

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Biochimie --- Biochimie clinique --- Biochemistry --- Molecular biology --- Cell biology --- Disease --- Xenobiotics --- Micronutrients --- Gene expression regulation --- physiopathology --- metabolism