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Lieber EXPERIMENTATOR, wir präsentieren Ihnen hier das Grundlagenwissen sowie Tipps und Tricks für den Umgang mit Nucleinsäuren. Sie werden sofort merken, dass der Autor Lust und Frust der täglichen Laborroutine genau kennt. Aus dem Inhalt: Präparieren, Fällen, Konzentrieren und Reinigen von Nucleinsäuren Das molekularbiologische Handwerkszeug (Restriktionsenzyme, Gele, Blotten) PCR (Polymerase-Kettenreaktion) RNA-Isolierung, -Transkription Klonierung von DNA-Fragmenten DNA-Nachweis und -Analyse (Markierung von Sonden, Hybridisierung, Screening, Sequenzierung) Modifikation und Funktionsanalyse von DNA-Sequenzen (Mutagenese, In-vitro-Translation, transgene Mäuse, Transgenexpression, Gentherapie, Genomik) Lieferantenverzeichnis Protokoll-Sammlungen gibt es viele, aber wer erklärt einem, was sich hinter den Methoden verbirgt? Dieses Buch richtet sich an alle Experimentatoren, die molekularbiologische Versuche durchführen wollen und gern nachvollziehen möchten, was sich in ihrem Reaktionsgefäß abspielt. Das ganze Spektrum der üblichen molekularbiologischen Methoden wird vorgestellt, kommentiert und Alternativen aufgezeigt. Und weil die Laborversuche nicht alles sind, findet sich auch noch ein Kapitel zum Thema Karriereplanung. Der lockere Ton wendet sich gleichermaßen an Studenten wie an BTAs und Laboranten, aber auch der alte Hase wird hier und dort noch etwas Neues entdecken. Die 7. Auflage wurde überarbeitet und aktualisiert. Über den Autor: Dr. Cornel Mülhardt. Geboren 1965. Biologie-Studium in Heidelberg und Hamburg. Wissenschaftlicher Angestellter in Erlangen (Institut für Med. Biochemie). Seit 1998 tätig bei Hoffmann-La Roche (Basel) in der Forschungsrichtung Neurobiologie. Seit Mai 2001 Leiter der Biologielaboranten-Ausbildung. Rezensionen der Vorauflagen: Wer sich einen realistischen Überblick über den Stand der Technik in der Molekularbiologie verschaffen will oder gar selber im Labor molekularbiologisch tätig werden (sein) möchte, muss dieses Buch lesen, chemikalienresistent einschlagen und ständig bei sich tragen! BioTec Zeigt Auswege ausexperimentellen Sackgassen und weckt ein Gespür für das richtige Experiment zur rechten Zeit. Bioworld.

Cell biology. --- Animal genetics. --- Microbial genetics. --- Microbial genomics. --- Proteomics. --- Cell Biology. --- Animal Genetics and Genomics. --- Microbial Genetics and Genomics.

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Groundbreaking research over the last 10 years has given rise to the hologenome concept of evolution. This concept posits that the holobiont (host plus all of its associated microorganisms) and its hologenome (sum of the genetic information of the host and its symbiotic microorganisms), acting in concert, function as a unique biological entity and therefore as a level of selection in evolution. All animals and plants harbor abundant and diverse microbiota, including viruses. Often the amount of symbiotic microorganisms and their combined genetic information far exceed that of their host. The microbiota with its microbiome, together with the host genome, can be transmitted from one generation to the next and thus propagate the unique properties of the holobiont. The microbial symbionts and the host interact in a cooperative way that affects the health of the holobiont within its environment. Beneficial microbiota protects against pathogens, provides essential nutrients, catabolizes complex polysaccharides, renders harmful chemicals inert, and contributes to the performance of the immune system. In humans and animals, the microbiota also plays a role in behavior. The sum of these cooperative interactions characterizes the holobiont as a unique biological entity. Genetic variation in the hologenome can be brought about by changes in either the host genome or the microbial population genomes (microbiome). Evolution by cooperation can occur by amplifying existing microbes, gaining novel microbiota and by acquiring microbial and viral genes. Under environmental stress, the microbiome can change more rapidly and in response to more processes than the host organism alone and thus influences the evolution of the holobiont. Prebiotics, probiotics, synbiotics and phage therapy are discussed as applied aspects of the hologenome concept.

Evolution (Biology). --- Life sciences. --- Microbial genomics. --- Microbiology. --- Zoology. --- Symbiogenesis --- Microbial genomics --- Evolution (Biology) --- Genomics --- Genetic Processes --- Eukaryota --- Biological Processes --- Biology --- Microbial Interactions --- Genome --- Organisms --- Biological Phenomena --- Biological Science Disciplines --- Genetic Structures --- Genetic Phenomena --- Microbiological Processes --- Phenomena and Processes --- Natural Science Disciplines --- Microbiological Phenomena --- Disciplines and Occupations --- Plants --- Microbiology --- Genetics --- Symbiosis --- Biological Evolution --- Metagenome --- Health & Biological Sciences --- Evolution --- Microbial biology --- Biosciences --- Sciences, Life --- Animal evolution --- Animals --- Biological evolution --- Darwinism --- Evolutionary biology --- Evolutionary science --- Origin of species --- Evolutionary biology. --- Life Sciences. --- Evolutionary Biology. --- Natural history --- Microorganisms --- Science --- Biological fitness --- Homoplasy --- Natural selection --- Phylogeny --- Microbial genetics

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In biochemistry, a metalloprotein is a generic term for a protein that contains a metal cofactor. The metal may be an isolated ion or may be coordinated with a nonprotein organic compound, such as the porphyrin found in hemoproteins. In some cases, the metal is co-coordinated with a side chain of the protein and an inorganic nonmetallic ion. This kind of protein-metal-nonmetal structure is seen in iron-sulfur clusters Metalloproteins deals with all aspects related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The biological roles of metal cations and metal-binding proteins are endless. They are involved in all crucial cellular activities. Many pathological conditions are related to the problematic metal metabolism. Research in metalloprotein-related topics is therefore rapidly growing, and different aspects of metal-binding proteins progressively enter curricula at Universities and even at the High School level on occasion. However, no key resource providing basic, but comprehensible knowledge on this rapidly expanding field exists. The Encyclopedia of Metalloproteins aims to bridge this gap, and will attempt to cover various aspects of metalloprotein/metalloproteomics and will deal with the different issues related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The goal is to cover exhaustively all catalytically and biologically crucial metal ions and to find at least one interacting protein for other metal ions. The Encyclopedia of Metalloproteins will provide a key resource for advanced undergraduate and graduate students, researchers, instructors, and professors interested in protein science, biochemistry, cell biology, and genetics.

Life sciences. --- Biochemistry. --- Proteomics. --- Cytology. --- Microbial genetics. --- Life Sciences. --- Biochemistry, general. --- Cell Biology. --- Microbial Genetics and Genomics. --- Proteins --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Metalloproteins --- Biology --- Chemistry --- Health & Biological Sciences --- Physical Sciences & Mathematics --- Biology - General --- Biochemistry --- Biosciences --- Sciences, Life --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Microorganisms --- Cell biology --- Cellular biology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Genetics --- Composition --- Cell biology. --- Microbial genomics. --- Microbiology --- Molecular biology --- Medical sciences --- Genomics --- Microbial genetics --- Cells --- Cytologists

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton. Continues the legacy of this premier serial with quality chapters authored by leaders in th

Genomics --- Genetic Techniques --- Biological Evolution --- Biochemistry --- Genetic Phenomena --- Classification --- Biological Science Disciplines --- Investigative Techniques --- Information Science --- Genetics --- Chemistry --- Computational Biology --- Phenomena and Processes --- Biological Processes --- Natural Science Disciplines --- Biological Phenomena --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biology --- Disciplines and Occupations --- Proteomics --- Phylogeny --- Sequence Analysis --- Metagenomics --- Gene Expression Profiling --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Microbial genomics. --- Metagenomics. --- Proteomics. --- RNA --- Research --- Methodology. --- Enzymes. --- Technique. --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology --- Enzymology.

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Pattern Formation in Morphogenesis is a rich source of interesting and challenging mathematical problems. The volume offers an interdisciplinary interaction space between biologists working in this field and mathematicians, who may propose solutions to the problems put forward by biologists. The main goal is to facilitate the process of cultivating a mutual recognition of the complementary skills between biologists and mathematicians, to the point where the resulting synergy generates new and novel discoveries in the field of Developmental Biology. Lastly, the volume shows how a combination of new discoveries in developmental biology and associated mathematical modeling and computational techniques has stimulated or may stimulate relevant advances in the field.                             .

Pattern formation (Biology) --- Morphogenesis. --- Morphogeny --- Organogenesis --- Biological pattern formation --- Mathematics. --- Bioinformatics. --- Microbial genetics. --- Microbial genomics. --- Applied mathematics. --- Engineering mathematics. --- Applications of Mathematics. --- Biomedicine general. --- Computational Biology/Bioinformatics. --- Microbial Genetics and Genomics. --- Mathematics, general. --- Embryology --- Morphology --- Developmental biology --- Medicine. --- Genomics --- Microbial genetics --- Microorganisms --- Genetics --- Microbiology --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Math --- Science --- Data processing --- Biomedicine, general. --- Health Workforce --- Engineering --- Engineering analysis --- Mathematical analysis --- Mathematics