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The objective of this Brief is to provide a solution to the unsolved technical problem in segmentation for the automated bone age assessment system. The task is accomplished by first applying the modified histogram equalized module, then applying the proposed automated anisotropic diffusion technique. It is followed by a novel fuzzy quadruple division scheme to optimize the central segmentation algorithm, and then an additional quality assurance scheme. The designed segmentation framework works without demanding scarce resources such as training sets and skillful operators.  The results have shown that the designed framework is capable of separating the soft-tissue and background from the hand bone with high accuracy. This Brief should be especially useful for students and professional researchers in the Biomedical and image processing fields.

Bones -- Growth. --- Bones -- Radiography. --- Skeletal maturity. --- Health & Biological Sciences --- Biomedical Engineering --- Bones --- Radiography. --- Growth. --- Bone age of human beings --- Maturity, Skeletal --- Skeletal age of human beings --- Bone --- Bone development --- Bone growth --- Osteogenesis --- Growth --- Engineering. --- Radiology. --- Medical physics. --- Radiation. --- Biomedical engineering. --- Biomedical Engineering. --- Signal, Image and Speech Processing. --- Imaging / Radiology. --- Medical and Radiation Physics. --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Physics --- Radiology --- Health physics --- Health radiation physics --- Medical radiation physics --- Radiotherapy physics --- Radiation therapy physics --- Radiological physics --- Radiation --- Construction --- Industrial arts --- Technology --- Age --- Human beings --- Human skeleton --- Age determination --- Diseases --- Radiology, Medical. --- Biomedical Engineering and Bioengineering. --- Clinical radiology --- Radiology, Medical --- Radiology (Medicine) --- Medical physics --- Signal processing. --- Image processing. --- Speech processing systems. --- Pictorial data processing --- Picture processing --- Processing, Image --- Imaging systems --- Optical data processing --- Computational linguistics --- Electronic systems --- Information theory --- Modulation theory --- Oral communication --- Speech --- Telecommunication --- Singing voice synthesizers --- Processing, Signal --- Information measurement --- Signal theory (Telecommunication)