Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The third edition of the Handbook of Proteolytic Enzymes is a fully revised and updated major reference work in Elsevier's canon. For the first time the Handbook will be available as an online via Elsevier's ScienceDirect platform as well as a three-volume book. The online version will have the enhanced options including online multimedia, cross-referencing capabilities, integrated online delivery and closer integration with the online MEROPS database of peptidases and their inhibitors. This reference work is intended for university libraries, researchers and students, and will be of great interest to the pharmaceutical and biotechnology companies. The new edition will feature articles on approximately 1000 different proteolytic enzymes written by acknowledged experts in the field. Each article will be a full but concise summary, including details of activity and specificity, structural chemistry, preparation and biological aspects. There are also introductory chapters on peptidase classification and mechanisms and a comprehensive index. Contains over 830 chapters. Covers new research in therapeutics and drug trialsSupplies content written by experts in the field.

Enzymology

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers fluorescence fluctuation spectroscopyContains chapte

Fluorescence. --- Luminescence --- Radioactivity --- Fluorescence spectroscopy. --- Fluctuations (Physics) --- Enzymology.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.

Biochemistry. --- Enzymes. --- Biochemistry, general. --- Enzymology. --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Is red wine good for you? And if so, why? How much? And what are the actual benefits? This addition to the SpringerBriefs in Cell Biology series thoroughly but succinctly answers these questions. It covers the biochemistry, health benefits and therapeutic potential of wine grapes.  It begins with  an overview of phytoalexin production in Vitis vinifera (Common Grape Vine), detailing the relationship of resveratrol to analogues such as pterostilbene, piceid and the viniferins (resveratrol oligomers).    The discussion then turns to the hundreds of reports linking resveratrol and related grape vine polyphenols to various beneficial health effects especially cardio- and cerebro- vascular, metabolic, anti-inflammatory and more.  Also addressed are the numerous intracellular mechanisms that have been shown to mediate the effects of these compounds in mammalian cells and tissues.  Finally, the authors discuss aspects of polyphenol bioavailability and how this will influence choices taken for delivering these compounds as nutritional supplements.  A brief chapter containing general conclusions and prospectus rounds out the information.

Histology. Cytology --- Enzymology --- General biochemistry --- Biology --- Biochemical engineering --- biochemie --- biologie --- biotechnologie --- cytologie --- histologie --- planten --- enzymen

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

In this thesis, Andrew Logsdail demonstrates that computational chemistry is a powerful tool in contemporary nanoscience, complementing experimental observations and helping guide future experiments. The aim of this particular PhD is to further our understanding of structural and compositional preferences in gold nanoparticles, as well as the compositional and chemical ordering preferences in bimetallic nanoalloys formed with other noble metals, such as palladium and platinum. Highlights include: calculations of the structural preferences and optical-response of gold nanoparticles and gold-containing nanoalloys; the design and implementation of novel numerical algorithms for the structural characterisation of gold nanoparticles from electron microscopy images; and electronic structure calculations investigating the interaction of gold nanoparticles with graphene and graphite substrates.The results presented here have significant implications for future research on the chemical and physical properties of gold-based nanoparticles and are of interest to many researchers working on experimental and theoretical aspects of nanoscience.

Chemical thermodynamics --- Chemical structure --- Chemistry --- Enzymology --- Computer. Automation --- katalyse --- moleculen --- thermodynamica --- nanotechniek --- biochemie --- chemie --- informatica --- biotechnologie --- atomen --- moleculaire biologie --- enzymen