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Embryology, Human --- Abnormalities, Human --- Embryonic Development --- Abnormalities --- Embryology --- Fetal Development --- methods --- Embryologie --- Vroedkunde --- Opleiding --- Embryology - methods

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Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.

Cytology. --- Embryology. --- Developmental biology. --- Cell Biology. --- Developmental Biology. --- Development (Biology) --- Biology --- Growth --- Ontogeny --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Embryology --- Development

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The Arthropoda is by far the largest living phylum, comprising over 1.2 million living species, and its unique evolutionary success is the primary focus for this up-to-date and comprehensive overview of the biology of the group.  This astonishing species richness is matched by a spectacular diversity in body forms and adaptations.  To counter the largely unavoidable trend towards increased specialization within a particular group, this volume adopts a comparative viewpoint across the entire phylum, encompassing both extant and fossil forms.  The phylum-wide perspective allows us to appreciate the wave of recent advances in knowledge of arthropod biology and evolution and to identify emerging themes and priorities for future research. As ever in the history of science, this wave of advances is driven by the rapid development of new methods and techniques.  New methods of extracting and studying fossils have vastly improved understanding of Palaeozoic arthropods.  New non-invasive, non-destructive techniques, such as micro-computed tomography, have revolutionised anatomical analysis and imaging. Arthropod comparative genomics is still in its infancy but high-throughput sequencing together with next-generation sequencing has facilitated spectacular growth in volumes of sequence data, which in turn has driven advances in bioinformatics. These novel methods have generated a wealth of data which has been critically reviewed by the chapter authors, to provide a new perspective on arthropod biology and evolution. The concise factual summaries and the questions articulated in this book will be of interest to evolutionary biologists, palaeontologists, developmental geneticists and invertebrate zoologists. It will be of special interest to advanced graduate and post-graduate students and have the potential to stimulate younger researchers to address questions in arthropod biology from the vantage point of a phylum-wide comparative perspective.

Arthropoda -- Development. --- Arthropoda -- Evolution. --- Arthropoda. --- Arthropoda --- Arthropoda, Fossil --- Biology --- Health & Biological Sciences --- Biophysics --- Development --- Evolution --- Physiology --- Development. --- Evolution. --- Arthropods --- Life sciences. --- Developmental biology. --- Embryology. --- Evolutionary biology. --- Invertebrates. --- Entomology. --- Life Sciences. --- Developmental Biology. --- Evolutionary Biology. --- Bilateria --- Invertebrates --- Evolution (Biology). --- Invertebrata --- Animals --- Insects --- Zoology --- Animal embryology --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Animal evolution --- Biological evolution --- Darwinism --- Evolutionary biology --- Evolutionary science --- Origin of species --- Biological fitness --- Homoplasy --- Natural selection --- Phylogeny --- Development (Biology) --- Growth --- Ontogeny --- Embryology

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Stem Cells Handbook, second edition, emphasizes the advances in understanding and working with embryonic and fetal stem cells as well as induced pluripotent stem cells that show the most promise for clinical use. This new edition has been completely rewritten and updated to reflect the significant advances made in this field since publication of the first edition in 2004. The thirty-six chapters in this book – all written by recognized experts in Stem Cell research – reflect the tremendous progress in the understanding of stem cell biology and the appreciation of the potential for using stem cells for therapy of cancer, aging, injury and congenital diseases. Arguably the biggest advances have been made in obtaining different populations of stem cells, inducing mature cells to act like stem cells, and the ever-critical issue of how to get these stem cells to expand and differentiate into the tissue precursor cells that then can be used to replace lost of damaged tissues. No less exciting are the recent advances in understanding how stem cells function to maintain tissue integrity and how to use this knowledge to prevent developmental abnormalities. Other key topics discussed include: properties of tissue stem cells , transplantation of stem cells, the stem cell as a target for cancer treatment and prevention.

Stem cells --- Medicine --- Cell Transplantation --- Biological Science Disciplines --- Cells --- Investigative Techniques --- Health Occupations --- Natural Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Transplantation --- Anatomy --- Surgical Procedures, Operative --- Disciplines and Occupations --- Stem Cell Transplantation --- Regenerative Medicine --- Stem Cells --- Methods --- Physiology --- Biology --- Health & Biological Sciences --- Cytology --- Stem cells. --- Cytology. --- Cell biology --- Cellular biology --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Life sciences. --- Regenerative medicine. --- Tissue engineering. --- Embryology. --- Life Sciences. --- Stem Cells. --- Regenerative Medicine/Tissue Engineering. --- Cytologists --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Embryology --- Development --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Regeneration (Biology)