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Cell death is an essential process in development, and a major contributor to a wide range of human diseases.  Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model systems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Necrosis: Methods and Protocols describes techniques  in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

Cytology. --- Cell cycle. --- Cell Biology. --- Cell Cycle Analysis. --- Apoptosis. --- Mitotic cycle --- Nuclear cycle (Cytology) --- Biological rhythms --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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Phenology refers to recurring plant and animal life cycle stages, such as leafing and flowering, maturation of agricultural plants, emergence of insects, and migration of birds. It is also the study of these recurring events, especially their timing and relationships with weather and climate. Phenological phenomena all give a ready measure of the environment as viewed by the associated organism, and are thus ideal indicators of the impact of local and global changes in weather and climate on the earth’s biosphere. Assessing our changing world is a complex task that requires close cooperation from experts in biology, climatology, ecology, geography, oceanography, remote sensing, and other areas. Like its predecessor, this second edition of Phenology is a synthesis of current phenological knowledge, designed as a primer on the field for global change and general scientists, students, and interested members of the public. With updated and new contributions from over fifty phenological experts, covering data collection, current research, methods, and applications, it demonstrates the accomplishments, progress over the last decade, and future potential of phenology as an integrative environmental science.

Phenology --- Botany --- Earth & Environmental Sciences --- Plant Ecology --- Phenology. --- Environmental sciences. --- Environmental science --- Life sciences. --- Atmospheric sciences. --- Plant ecology. --- Zoology. --- Climate change. --- Ecotoxicology. --- Life Sciences. --- Plant Ecology. --- Climate Change. --- Atmospheric Sciences. --- Science --- Bioclimatology --- Biological rhythms --- Climatic changes. --- Environmental toxicology. --- Biology --- Natural history --- Animals --- Ecotoxicology --- Pollutants --- Pollution --- Environmental health --- Toxicology --- Changes, Climatic --- Changes in climate --- Climate change --- Climate change science --- Climate changes --- Climate variations --- Climatic change --- Climatic changes --- Climatic fluctuations --- Climatic variations --- Global climate changes --- Global climatic changes --- Climatology --- Climate change mitigation --- Teleconnections (Climatology) --- Plants --- Ecology --- Environmental aspects --- Phytoecology --- Vegetation ecology --- Atmospheric sciences --- Earth sciences --- Atmosphere --- Floristic ecology --- Global environmental change

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The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.

Cytology. --- Cell cycle. --- Cell Biology. --- Cell Cycle Analysis. --- Apoptosis. --- Mitotic cycle --- Nuclear cycle (Cytology) --- Biological rhythms --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Aging --- Biomarkers. --- Cellular Senescence --- physiology. --- Biochemical Marker --- Biologic Marker --- Biologic Markers --- Clinical Marker --- Immune Marker --- Immune Markers --- Immunologic Marker --- Laboratory Marker --- Marker, Biochemical --- Marker, Biological --- Marker, Clinical --- Marker, Immunologic --- Marker, Laboratory --- Marker, Serum --- Marker, Surrogate --- Serum Marker --- Surrogate End Point --- Surrogate End Points --- Surrogate Endpoint --- Surrogate Endpoints --- Surrogate Marker --- Viral Marker --- Biochemical Markers --- Biological Markers --- Clinical Markers --- Immunologic Markers --- Laboratory Markers --- Markers, Biochemical --- Markers, Biological --- Markers, Clinical --- Markers, Immunologic --- Markers, Laboratory --- Markers, Serum --- Markers, Surrogate --- Markers, Viral --- Serum Markers --- Surrogate Markers --- Viral Markers --- Biological Marker --- End Point, Surrogate --- End Points, Surrogate --- Endpoint, Surrogate --- Endpoints, Surrogate --- Marker, Biologic --- Marker, Immune --- Marker, Viral --- Markers, Biologic --- Markers, Immune --- Molecular Probes --- Endophenotypes --- Biomarker