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Increasing knowledge of the biological is fundamentally transforming what life itself means and where its boundaries lie. New developments in the biosciences - especially through the molecularisation of life - are (re)shaping healthcare and other aspects of our society. This cutting edge volume studies contemporary bio-objects, or the categories, materialities and processes that are central to the configuring of 'life' today, as they emerge, stabilize and circulate through society. Examining a variety of bio-objects in contexts beyond the laboratory, Bio-Objects: Life in the 21st Century explores new ways of thinking about how novel bio-objects enter contemporary life, analysing the manner in which, among others, the boundaries between human and animal, organic and non-organic, and being 'alive' and the suspension of living, are questioned, destabilised and in some cases re-established. Thematically organised around questions of changing boundaries; the governance and regulation of bio-objects; and changing social, economic and political relations, this book presents rich new case studies from Europe that will be of interest to scholars of science and technology studies, social theory, sociology and law.

Sociology of knowledge --- Professional ethics. Deontology --- Europe --- Genetic engineering. --- Life (Biology). --- Life sciences --- Social aspects. --- Sciences de la vie --- Vie (biologie) --- Animaux transgéniques --- Génie génétique --- Organismes transgéniques --- Bioéthique. --- Biotechnologie --- Transgenic animals --- Genetic Engineering --- Organisms, Genetically Modified --- Biological Science Disciplines --- Aspect social --- Philosophie --- Éthique --- Social aspects --- ethics --- Organisms, Genetically Modified. --- ethics. --- Animaux transgéniques. --- Organismes transgéniques. --- Aspect social. --- Philosophie. --- Éthique. --- Life sciences - Social aspects --- Droit médical --- Animaux transgéniques. --- Génie génétique --- Organismes transgéniques. --- Bioéthique. --- Éthique.

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The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

Transgenic mice --- Animals, Genetically Modified --- Investigative Techniques --- Mice --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Murinae --- Animal Population Groups --- Animals --- Muridae --- Eukaryota --- Rodentia --- Mammals --- Organisms --- Vertebrates --- Chordata --- Mice, Transgenic --- Genetic Techniques --- Biology --- Health & Biological Sciences --- Genetics --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Founder Mice, Transgenic --- Mouse, Founder, Transgenic --- Mouse, Transgenic --- Transgenic Mice --- Mice, Transgenic Founder --- Transgenic Founder Mice --- Transgenic Mouse --- Chordates --- Chordate --- Vertebrate --- Mammalia --- Mammal --- Rodents --- Beavers --- Capybaras --- Castor Beaver --- Dipodidae --- Hydrochaeris --- Jerboas --- Beaver --- Capybara --- Hydrochaeri --- Jerboa --- Rodent --- Rodentias --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Murids --- Murid --- Animalia --- Animal --- Metazoa --- Animal Population Group --- Population Group, Animal --- Population Groups, Animal --- Acomys --- Apodemus --- Bandicoot Rats --- Bandicota --- Mastomys --- Praomys --- Rats, Bandicoot --- Bandicoot Rat --- Bandicotas --- Rat, Bandicoot --- Mice, Laboratory --- Mouse --- Mouse, House --- Mouse, Laboratory --- Mouse, Swiss --- Mus domesticus --- Mus musculus domesticus --- Swiss Mice --- Mice, House --- Mus --- Mus musculus --- House Mice --- House Mouse --- Laboratory Mice --- Laboratory Mouse --- Mice, Swiss --- Swiss Mouse --- domesticus, Mus musculus --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Founder Animals, Transgenic --- GMO Animals --- Genetically Engineered Animals --- Animals, Transgenic --- Genetically Modified Animals --- Transgenic Animals --- Animal, GMO --- Animal, Genetically Engineered --- Animal, Genetically Modified --- Animal, Transgenic --- Animal, Transgenic Founder --- Animals, GMO --- Animals, Genetically Engineered --- Animals, Transgenic Founder --- Engineered Animal, Genetically --- Engineered Animals, Genetically --- Founder Animal, Transgenic --- GMO Animal --- Genetically Engineered Animal --- Genetically Modified Animal --- Modified Animal, Genetically --- Modified Animals, Genetically --- Transgenic Animal --- Transgenic Founder Animal --- Transgenic Founder Animals --- methods --- Animal genetics. --- Genetic engineering. --- Medical genetics. --- Animal Genetics and Genomics. --- Genetic Engineering. --- Gene Function. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects --- Transgenic mice - Laboratory manuals