Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Presenting an area of research that intersects with and integrates diverse disciplines, including molecular biology, applied informatics, and statistics, among others, Bioinformatics for Omics Data: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study.  Divided into three convenient sections, this detailed volume covers central analysis strategies, standardization and data-management guidelines, and fundamental statistics for analyzing Omics profiles, followed by a section on bioinformatics approaches for specific Omics tracks, spanning genome, transcriptome, proteome, and metabolome levels, as well as an assortment of examples of integrated Omics bioinformatics applications, complemented by case studies on biomarker and target identification in the context of human disease.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Bioinformatics for Omics Data: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.

Bioinformatics --- Genomics --- Computational biology --- Computational Biology --- Data processing --- methods --- Investigative Techniques --- Biology --- Genetics --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- Methods --- Health & Biological Sciences --- Biophysics --- Biology - General --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Bio-Informatics --- Biology, Computational --- Computational Molecular Biology --- Molecular Biology, Computational --- Bio Informatics --- Bio-Informatic --- Bioinformatic --- Biologies, Computational Molecular --- Biology, Computational Molecular --- Computational Molecular Biologies --- Molecular Biologies, Computational --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genome research --- Genomes --- Bio-informatics --- Biological informatics --- Research --- Bioinformatics. --- Biological models. --- Systems Biology. --- Computer Appl. in Life Sciences. --- Models, Biological --- Information science --- Systems biology --- Data processing. --- Genomics - Data processing --- Computational Biology - methods

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Transgenic animals --- Genetic engineering --- Genetic Engineering --- Animals, Genetically Modified --- Methodology --- methods --- Investigative Techniques --- Organisms, Genetically Modified --- Publication Formats --- Animal Population Groups --- Genetic Techniques --- Animals --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Organism Forms --- Publication Characteristics --- Eukaryota --- Organisms --- Laboratory Manuals --- Methods --- Biology --- Health & Biological Sciences --- Genetics --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Founder Animals, Transgenic --- GMO Animals --- Genetically Engineered Animals --- Animals, Transgenic --- Genetically Modified Animals --- Transgenic Animals --- Animal, GMO --- Animal, Genetically Engineered --- Animal, Genetically Modified --- Animal, Transgenic --- Animal, Transgenic Founder --- Animals, GMO --- Animals, Genetically Engineered --- Animals, Transgenic Founder --- Engineered Animal, Genetically --- Engineered Animals, Genetically --- Founder Animal, Transgenic --- GMO Animal --- Genetically Engineered Animal --- Genetically Modified Animal --- Modified Animal, Genetically --- Modified Animals, Genetically --- Transgenic Animal --- Transgenic Founder Animal --- Transgenic Founder Animals --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Animalia --- Animal --- Metazoa --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Animal Population Group --- Population Group, Animal --- Population Groups, Animal --- GMO Organisms --- Genetically Engineered Organisms --- Genetically Modified Organisms --- Organisms, Transgenic --- Transgenic Organisms --- Engineered Organism, Genetically --- Engineered Organisms, Genetically --- GMO Organism --- Genetically Engineered Organism --- Genetically Modified Organism --- Modified Organism, Genetically --- Modified Organisms, Genetically --- Organism, GMO --- Organism, Genetically Engineered --- Organism, Genetically Modified --- Organism, Transgenic --- Organisms, GMO --- Organisms, Genetically Engineered --- Transgenic Organism --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Designed genetic change --- Gene splicing --- Genetic intervention --- Genetic surgery --- Life sciences. --- Animal genetics. --- Transgenic organisms. --- Life Sciences. --- Animal Genetics and Genomics. --- Transgenics. --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Biosciences --- Sciences, Life --- Science

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.

Human histology. Human cytology --- Chemical laboratory practice --- Tissues --- Tissue culture --- Diagnostic immunohistochemistry --- Tissue Embedding --- Genetic Techniques --- Microarray Analysis --- Culture Techniques --- Publication Formats --- Investigative Techniques --- Clinical Laboratory Techniques --- Histocytological Preparation Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Microchip Analytical Procedures --- Cytological Techniques --- Chemistry Techniques, Analytical --- Histological Techniques --- Gene Expression Profiling --- Laboratory Manuals --- Paraffin Embedding --- Tissue Array Analysis --- Methods --- Tissue Culture Techniques --- Human Anatomy & Physiology --- Biology --- Health & Biological Sciences --- Physiology --- Biophysics --- Analysis --- Embedding, Paraffin --- Gene Expression Monitoring --- Transcriptome Analysis --- Gene Expression Pattern Analysis --- Transcript Expression Analysis --- Transcriptome Profiling --- mRNA Differential Display --- Analyses, Transcript Expression --- Analyses, Transcriptome --- Analysis, Transcript Expression --- Analysis, Transcriptome --- Differential Display, mRNA --- Differential Displays, mRNA --- Display, mRNA Differential --- Displays, mRNA Differential --- Gene Expression Monitorings --- Gene Expression Profilings --- Monitoring, Gene Expression --- Monitorings, Gene Expression --- Profiling, Gene Expression --- Profiling, Transcriptome --- Profilings, Gene Expression --- Profilings, Transcriptome --- Transcript Expression Analyses --- Transcriptome Analyses --- Transcriptome Profilings --- mRNA Differential Displays --- Histologic Technic --- Histologic Technics --- Histologic Technique --- Histologic Techniques --- Histological Technics --- Technic, Histologic --- Technics, Histologic --- Technique, Histologic --- Techniques, Histologic --- Histological Technic --- Histological Technique --- Technic, Histological --- Technics, Histological --- Technique, Histological --- Techniques, Histological --- Histology --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Microchip Analysis --- Analyses, Microchip --- Analysis, Microchip --- Analytical Procedure, Microchip --- Analytical Procedures, Microchip --- Microchip Analytical Procedure --- Procedure, Microchip Analytical --- Procedures, Microchip Analytical --- Cytohistologic Preparation Technics --- Cytohistologic Preparation Technique --- Cytohistologic Preparation Techniques --- Cytohistological Preparation Technic --- Cytohistological Preparation Technics --- Cytohistological Preparation Technique --- Histocytologic Preparation Technic --- Histocytologic Preparation Technics --- Histocytologic Preparation Technique --- Histocytologic Preparation Techniques --- Histocytological Preparation Technic --- Histocytological Preparation Technics --- Histocytological Preparation Technique --- Preparation Technic, Cytohistologic --- Preparation Technic, Cytohistological --- Preparation Technic, Histocytologic --- Preparation Technic, Histocytological --- Preparation Technics, Cytohistologic --- Preparation Technics, Cytohistological --- Preparation Technics, Histocytologic --- Preparation Technics, Histocytological --- Preparation Technique, Cytohistologic --- Preparation Technique, Cytohistological --- Preparation Technique, Histocytologic --- Preparation Technique, Histocytological --- Preparation Techniques, Cytohistologic --- Preparation Techniques, Cytohistological --- Preparation Techniques, Histocytologic --- Preparation Techniques, Histocytological --- Technic, Cytohistologic Preparation --- Technic, Cytohistological Preparation --- Technic, Histocytologic Preparation --- Technic, Histocytological Preparation --- Technics, Cytohistologic Preparation --- Technics, Cytohistological Preparation --- Technics, Histocytologic Preparation --- Technics, Histocytological Preparation --- Technique, Cytohistologic Preparation --- Technique, Cytohistological Preparation --- Technique, Histocytologic Preparation --- Technique, Histocytological Preparation --- Techniques, Cytohistologic Preparation --- Techniques, Cytohistological Preparation --- Techniques, Histocytologic Preparation --- Techniques, Histocytological Preparation --- Cytohistological Preparation Techniques --- Cytohistologic Preparation Technic --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Microarray Analytical Devices --- Microarray Microchips --- Nanoarray Analytical Devices --- Analysis, Microarray --- Analytical Device, Microarray --- Analytical Device, Nanoarray --- Analytical Devices, Microarray --- Analytical Devices, Nanoarray --- Device, Microarray Analytical --- Device, Nanoarray Analytical --- Devices, Microarray Analytical --- Devices, Nanoarray Analytical --- Microarray Analytical Device --- Microarray Microchip --- Microchip, Microarray --- Microchips, Microarray --- Nanoarray Analytical Device --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Diagnostic immunohistology --- Culture Technique, Tissue --- Culture Techniques, Tissue --- Tissue Culture Technique --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- methods --- Cultures and culture media --- Cell Array Analysis --- Cell Microarray Analysis --- Tissue Microarray Analysis --- Analyses, Cell Array --- Analyses, Cell Microarray --- Analyses, Tissue Array --- Analyses, Tissue Microarray --- Analysis, Cell Array --- Analysis, Cell Microarray --- Analysis, Tissue Array --- Analysis, Tissue Microarray --- Array Analyses, Cell --- Array Analyses, Tissue --- Array Analysis, Cell --- Array Analysis, Tissue --- Cell Array Analyses --- Cell Microarray Analyses --- Microarray Analyses, Cell --- Microarray Analyses, Tissue --- Microarray Analysis, Cell --- Microarray Analysis, Tissue --- Tissue Array Analyses --- Tissue Microarray Analyses --- Embedding, Tissue --- Medicine. --- Pathology. --- Molecular Medicine. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Health Workforce