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Each lesson is an experiment designed to help students understand biotechnology's applications in agriculture, environmental protection, medicine, and manufacturing. Throughout the book, they'll explore how biotechnology is as venerable a process as the making of cheese, and as new a technique as extracting DNA from living tissue.  They'll also investigate bioethics and examine possible careers in the field.

- Use *Hands-On Science: Introduction to Biotechnology* as a complete introductory program, as a replacement unit, or as an enrichment experience. 
- Each activity includes teacher notes, materials lists, and assessments. 
- *Hands-On Science: Introduction to Biotechnology* correlates to National Science Education standards.


Biotechnology --- gisten --- boter --- fermentatie --- gelelektroforese --- antibiotica --- biotechnologie --- DNA (deoxyribonucleic acid) --- electrophoresis --- klonen --- kaas --- enzymen

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Preface 
1. INTRODUCTION TO MOLECULAR DIAGNOSTICS 
1.1 Prologue 
1.2 Clinical diagnostics entering a new phase 
1.3 Concept of Molecular Diagnostics 
1.4 Molecular Diagnostic Technology and Health Care Industries 
1.5 Molecular diagnostic test 
1.6 Conclusion 
2. OMICS TECHNOLOGY 
2.1 Prologue 
2.2 Concept of Omics 
2.3 Genome and Genomics 
2.4 Transcriptome and Transcriptomics 
2.5 Proteome and Proteomics 
2.6 Metabolome and Metabolomics 
2.7 Integration of Omic 
2.8 Conclusion 
3. RECOMBINANT DNA PHARMACEUTICALS 
3.1 Prologue 
3.2 Concept 
3.3 Tools of Recombinant DNA Technology 
3.4 Therapeutic Applications of Recombinant DNA derived Pharmaceuticals 
3.5 Conclusion 
4. APTAMERS: IN VITRO DNA SELECTION 
4.1 Prologue 
4.2 Concept 
4.3 Selection of Aptamers 
4.4 Applications of DNA and RNA Aptamers 
4.5 conclusion 
5. REPORTER GENE 
5.1 Prologue 
5.2 Concept 
5.3 Reporter Gene Assay 
5.4 Transformation and Transfection Assays 
5.5 Gene Expression Assays 
5.6 Promoter Assays 
5.7 Role of Reporter Gene Assay in Molecular Diagnostics 
5.8 Conclusion 
6. IMPACT OF HCP ON MOLECULAR DIAGNOSTICS 
6.1 Prologue 
6.2 Origin of the Human Genome Project 
6.3 Goals and Issues of HGP 
6.4 The Human Genome and Genetic Variation 
6.5 Human Genome Project and Health Services and Improve Health 6.6 Single Gene Disease 
6.7 New and Individualised Drug Treatments 
6.8 The Achievements and Challenges of The HGP 
6.9 Information Transfer 
6.10 Impact of the Human Genome Project on Epidemiologic Research 
6.11 Impact of the Human Genome Project on Our Genomic Makeup 6.12 Conclusion 
7. MOLECULAR DIAGNOSIS IN THE POST GENOMIC AND PROTEOMIC ERA 
7.1 Prologue 
7.2 The Genomic Era 
7.3 Concept 
7.4 The Post Genomic Era 
7.5 Advantages of Combining Multiple Types of Data 
7.5 Biomedical Research in the Postgenomic Era 7.6 Conclusion 
8. ETHICS, PATENTS AND REGULATIONS 
8.1 Prologue 
8.2 Concept of Ethical, Legal, and Social Issues 
8.3 Ethical and Social Issues in Diagnostic Molecular Genetics 
8.4 Confronting the Ethical, Legal and Social Issues 
8.5 Genes and Disease 
8.6 The Existence of Genetic Information 
8.7 Impact of the Human Genome Project at the Interface between Patent and FDA Law 
8.8 Regulatory Process 
8.9 Conclusion 
9. POLYMERIZED CHAIN REACTION 
9.1 Prologue 
9.2 The Concept 
9.3 PCR Optimisation 
9.4 Advances in the PCR Technique 
9.5 Cloning PCR Products 
9.6 PCR as a Diagnostic Tool 
9.7 Conclusion 
10. IN SITU HYBRIDISATION 
10.1 Prologue 
10.2 The Concept of in SITU Hybridisation 
10.3 Disadvantages of Radioactive Probes 
10.4 Solving the Problem: Advent of Fish Technique 
10.5 Applications of Fish as a Diagnostic Tool for Research 
10.6 Recent Advances of in SITU Hybridisation Technology 
10.7 Conclusion 
11. IMMUNOASSAY ELISA 
11.1 Prologue 
11.2 Concept 
11.3 Types of Immunoassay 
11.4 Recent advances in the Field of Immunodiagnostics 
11.5 Clinical Applications of Immunoassay 
11.6 Utilization and Interpretation of Immunological Tests 
11.7 Conclusion 
12. PHAGE DISPLAY 
12.1 Prologue 
12.2 Concept 
12.3 Phage-Display Libraries as Populations of Replicable, Mutable Chemical 
12.4 Practical Applications of Phage Display 
12.5 Conclusion 
13. MICROARRAY 
14. DNA BIOSENSORS 
15. MOLECULAR MICROBIOLOGICAL TESTING 
16. PTOTEOMIC TECHNOLOGY 
17. NANOMEDICINE 
18. BIOMARKERS 
19. DIAGNOSIS AND MONITORING OF INFECTIONS 
20. DIAGNOSIS OF MUTATION AND GENETIC DISORDERS 
21. DIAGNOSIS OF COMPLEX DISEASES 
22. BIOCHIPS 
23. PERSONALISED MEDICINE 
24. BIOPHARMACEUTICAL INDUSTRY AND HEALTH CARE 
25. FORENSIC MEDICINE 
26. PHARMACOGENOMICS 
27. GENE TECHNOLOGY IN FORENSIC SCIENCES 
28. FOOD DIAGNOSTICS 
29. RAPID DIAGNOSTIC METHODS FOR BIOWARFARE 
30. SEGMENTS OF MOLECULAR DIAGNOSTICS : MARKET PLACE

 A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.

Macromolecules --- Molecular biology --- PCR (polymerase chain reaction) --- klinische chemie --- recombinant DNA --- in situ hybridisatie --- biosensoren --- proteomics --- genomics --- microarrays --- biogeneesmiddelen --- klonen --- polymeren --- moleculaire biologie --- proefdieren

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(Publisher-supplied data) Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. " ... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections ... there is a Further Reading section after each chapter that contains several key references ... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." --Journal of Heredity, 2007 (on the previous edition)

klonen --- polymers --- yeast [fungi] --- biotechnologie --- Biotechnology --- genetica --- gisten --- polymeren --- recombinant DNA --- Macromolecules --- Genetics --- Biotechnologie --- Gentechnologie --- Recombinant DNA-technologie --- 575.1 --- Klonering --- Genetica --- DNA --- Molecular cloning. --- Nucleotide sequence. --- Cloning, Molecular. --- DNA, Recombinant --- Sequence Analysis, DNA. --- Analysis. --- analysis. --- Cloning, molecular. --- Dna --- Dna, recombinant --- Sequence analysis, dna. --- Molecular cloning --- Nucleotide sequence --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Nucleic acids --- Nucleotides --- Sequence alignment (Bioinformatics) --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Analysis --- moleculaire biologie --- Escherichia coli --- proteomics --- enzymen --- Ingeniería Genética (49152202) --- Ingeniería Genética y Genómica (70981102) --- Bibliografía recomendada --- Escherichia --- PCR (polymerase chain reaction)