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In tumors, cells located at some distance from blood vessels have to face a very hypoxic microenvironment. Hypoxic cells use glycolysis to produce energy, thereby creating a lactate gradient opposite to the oxygen gradient. Although lactate is generally considered as a metabolic waste product, it was recently documented in the FATH pole that lactate can also act as a substrate fueling the oxidative metabolism of oxygenated cells. In this study, we show that lactate is not only an energy substrate but takes also an active part in cancer development. More precisely, we found that lactate promotes tumor cell migration and stimulates expression of key actors for tumor invasiveness, including A1lvf P2 and ezrin. A correlation between lactate levels and angiogenesis was also established by comparing the vascular phenotype of oxidative and glycolytic tumors. These findings identify lactate as a key mediator of signaling pathways supporting cancer progression. Au sein d'une tumeur, certaines cellules situées à distance des vaisseaux sanguins doivent faire face à un microenvironnement très hypoxique.Ces cellules utilisent la glycolyse pour la production d'énergie créant un gradient de lactate inverse au gradient d'oxygène.Bien que le lactate soit généralement considéré comme un déchet du métabolisme, des recherches menées au laboratoire FATH ont montré qu'il peut également agir comme un substrat qui alimente le métabolisme oxydatif des cellules oxygénées. Dans cette étude, nous montrons que le lactate n'est pas seulement un substrat énergétique mais est également un médiateur de l'agressivité tumorale.Plus précisément, nous décrivons que le lactate favorise la migration des cellules tu morales et stimule l'expression d'acteurs clés de l 'invasivité tumorale, tel que la MMP2 et l'ezrin.Une corrélation entre taux de lactate et angiogenèse a également pu être établie en comparant le profil vasculaire de tumeurs oxydatives et glycolytiques. Ces découvertes établissent pour le lactate, un rôle de médiateur de certaines voies signalétiques favorables à la progression du cancer.

Lactates --- Tumor Cells, Cultured --- Phenotypes

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This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture

Histology. Cytology --- cytologie --- dierlijke cellenkweek --- medische laboratoriumtechnologie --- Tissue culture --- Cell culture --- 619.08 --- Diseases of domesticated animals. Veterinary science--?.08 --- 619.08 Diseases of domesticated animals. Veterinary science--?.08 --- Tissues --- Cultures (Biology) --- Organ culture --- Cytology --- Cultures and culture media --- Technique --- stamcellen --- klonen --- moleculaire biologie --- ophanging (autotechnologie) --- tumoren --- enzymen --- Tissue culture techniques --- Cell culture techniques --- Cell physiological processes --- Cells, cultured --- Germ cells --- Cell separation --- Cell biology --- Tissue preservation --- Stem cells --- Cells, culture --- Bioethics --- mehtods --- physiology

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Animal cell technology is a growing discipline of cell biology which aims not only to understand the structure, function and behavior of differentiated animal cells, but also to ascertain their ability to be used for industrial and medical purposes. Some of the major goals of animal cell technology include: the clonal expansion of differentiated cells, the optimization of their culture conditions, modulation of their ability for the production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods. This volume gives the readers a complete review of the present state-of-the-art research in Japan and other countries where this field is well advanced. The Proceedings will be useful to cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and to those working in either academic environments or in the biotechnology and pharmacy industries related to animal cell culture.

Animal cell biotechnology -- Congresses. --- Biological products -- Congresses. --- Animal cell biotechnology --- Cells --- Publication Formats --- Technology --- Complex Mixtures --- Culture Techniques --- Biological Science Disciplines --- Cell Culture Techniques --- Biotechnology --- Cells, Cultured --- Congresses --- Biological Products --- Chemicals and Drugs --- Clinical Laboratory Techniques --- Natural Science Disciplines --- Anatomy --- Technology, Industry, and Agriculture --- Publication Characteristics --- Technology, Industry, Agriculture --- Disciplines and Occupations --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Mechanical Engineering --- Biology --- Bioengineering --- Cytology --- Health & Biological Sciences --- Engineering & Applied Sciences --- Chemistry. --- Biotechnology. --- Food --- Life sciences. --- Cell biology. --- Biomedicine general. --- Cell Biology. --- Life Sciences, general. --- Food Science.