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Quantitative methods in social research --- Mathematical statistics --- Biological techniques --- Social sciences --- Biology --- Optimal designs (Statistics) --- Research --- maatschappijwetenschappen, methoden --- regressie-analyse --- wiskundige functies --- Social science research --- Experimental design --- Biological research --- Biomedical research --- Social sciences - Research --- Biology - Research

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Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.

Immunology. --- Cytology. --- Medical laboratories. --- Cell Biology. --- Laboratory Medicine. --- Immunobiology --- Life sciences --- Serology --- Diagnosis, Laboratory --- Health facilities --- Laboratories --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Dendritic cells - Laboratory manuals --- Medicine --- Biomedical Research. --- Health Workforce --- Biological research --- Biomedical research --- Research. --- Dendritic cells

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Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

Cell lines. --- Cell culture. --- Cultures (Biology) --- Cytology --- Cell culture --- Technique --- Medicine. --- Cytology. --- Genetic engineering. --- Biochemistry. --- Biomedicine general. --- Cell Biology. --- Genetic Engineering. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Composition --- Cell biology. --- Biomedicine, general. --- Health Workforce --- Medicine --- Biotechnology. --- Biomedical Research. --- Chemical Bioengineering. --- Research. --- Chemical engineering --- Genetic engineering --- Biological research --- Biomedical research

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The Leydig cells of the testis represent the main source of androgens. The idea of Leydig cells as endocrine cells has been the leading characteristic of this interesting cell population till now. Our studies of the last 2 decades allowed us to reveal a new important feature of Leydig cells that is their obvious similarity with structures of the central and peripheral nervous system. This includes the expression of neurohormones, neurotransmitters, neuropeptides and glial cell antigens. In this way, it became evident that in addition to the well established control by steroids and systemic hormones, important local auto- and paracrine control mechanisms of testicular functions exist. Thus, the Leydig cells represent a specialized cell population with both endocrine and neuroendocrine properties. The discovery of the neuroendocrine features of Leydig cells gave rise to the hypothesis of a potential neuroectodermal and/or neural crest origin of testicular Leydig cells. In an experimental animal model we revealed that adult Leydig cells originate by transdifferentiation from stem/progenitor cells (pericytes and smooth muscle cells), underlying the close relationship of Leydig cells with testis microvasculature. This and the supporting data from the literature provided the basis for revealing the pericytes as a common adult stem cell type of mammalian species. Distributed by the microvasculature through the entire body, the pericyte, acting as a resting early pluripotent adult stem cell, provides an ingenious system to assure the maintenance, physiological repair and regeneration of organs, each under the influence of specific local environmental factors.

Leydig cells. --- Paraneurons. --- Stem cells. --- Leydig cells --- Paraneurons --- Stem cells --- Pericytes --- Leydig Cells --- Stem Cells --- Endothelium, Vascular --- Epithelial Cells --- Cells --- Mesoderm --- Testis --- Endocrine Cells --- Genitalia, Male --- Blood Vessels --- Gonads --- Anatomy --- Endothelium --- Germ Layers --- Epithelium --- Embryonic Structures --- Genitalia --- Cardiovascular System --- Endocrine Glands --- Tissues --- Urogenital System --- Endocrine System --- Physiology --- Human Anatomy & Physiology --- Health & Biological Sciences --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Neuroendocrine cells --- Interstitial cells of Leydig --- Leydig's cells --- Testicular interstitial cells --- Medicine. --- Endocrinology. --- Biomedicine. --- Biomedicine general. --- Neurons --- Chromaffin cells --- Internal medicine --- Hormones --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Health Workforce --- Endocrinology . --- Biomedicine, general. --- Medicine --- Biology --- Biomedical Research. --- Research. --- Biological research --- Biomedical research

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Ischemic brain damage represents a major source of morbidity and mortality in westernized society and poses a significant financial burden on the health care system. To date, few effective therapies have been realized to treat stroke and promising avenues have not proven clinically useful. Recent evidence, however, suggests that channels, pumps, and ionic exchangers are involved in CNS ischemia and ischemic stroke, but the potential contribution of these channels for curing stroke is far less understood than for many other normal and pathological conditions. New Strategies in Stroke Intervention: Ionic Channels, Pumps, and Transporters analyzes the roles played by targets in stroke development and the potential action of drugs modulating these proteins. This book provides a groundbreaking review of these ionic channels, pumps, and transporters as regulators of neuronal ionic homeostasis, providing a better understanding of ischemic brain disorders and the new pharmacological avenues for a cure. It will be a useful tool for researchers working in this field, and any student interested in the physiological, pathophysiological, and pharmacological features of stroke damage. About the Editor: Lucio Annunziato is a professor of pharmacology in the School of Medicine at the University of Naples ‘‘Federico II’’, Italy. He is coordinator of the University's neuroscience Ph.D. program and director of the Department of Neuroscience. He is also the president elect of the Italian Society of Neuroscience. Dr. Annunziato has authored more than 150 full papers and his scientific interest is mainly devoted to the molecular pharmacology of plasma membrane transporters and ionic channels involved in the pathophysiology of stroke. Most of his recent papers have appeared in the most qualified international journals in the field of ischemic neurodegeneration.

Cerebrovascular disease --- Brain --- Cerebrum --- Mind --- Treatment. --- Diseases --- Medicine. --- Molecular biology. --- Neurosciences. --- Pharmacology. --- Anesthesiology. --- Cardiology. --- Neurology. --- Biomedicine. --- Molecular Medicine. --- Pharmacology/Toxicology. --- Central nervous system --- Head --- Toxicology. --- Neuroscience. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Heart --- Internal medicine --- Medicine --- Neuropsychiatry --- Anaesthesiology --- Surgery --- Chemicals --- Pharmacology --- Poisoning --- Poisons --- Toxicology --- Health Workforce --- Neurology . --- Drug effects --- Medical pharmacology --- Chemotherapy --- Drugs --- Pharmacy --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Physiological effect --- Biology --- Biomedical Research. --- Research. --- Biological research --- Biomedical research