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One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.
Genetic engineering. --- Human genetics. --- Animal genetics. --- Transgenic organisms. --- Genetic Engineering. --- Human Genetics. --- Animal Genetics and Genomics. --- Transgenics. --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Organisms --- Genetic engineering --- Genetics --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Heredity, Human --- Human biology --- Physical anthropology
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National, European and international concepts and strategies concerning the legal and ethical framework of chimera and hybrid research are still largely missing, even though they are absolutely necessary in order to use the potential of chimera and hybrid research effectively and efficiently for the benefit of science and society. The outcome of the CHIMBRIDS-Project successfully sheds light on the chances and risks of this research and provides legal solutions to existing problems in order to help decision-makers fulfil their tasks in an informed and efficient manner. This comprehensive volume details the complete results, contributed by 40 scholars from 10 member states of the European Union, Canada, China, Israel, Japan, Switzerland and the US, with descriptive reports of the legal situation in specific countries and in-depth analysis of all scientific, medical, ethical and legal implications of chimera and hybrid research.
CHIMBRIDS-project.
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Genetic engineering --Research.
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Mosaicism --Research.
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Transgenic animals --Research.
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Mosaicism
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Transgenic animals
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Genetic engineering
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Internationality
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Animals, Genetically Modified
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Chimera
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Ethics, Research
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Biomedical Research
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Genetic Engineering
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Research
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Ethics
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Organism Forms
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Plants
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Organisms, Genetically Modified
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Animal Population Groups
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Genetic Techniques
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Social Sciences
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Humanities
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Investigative Techniques
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Animals
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Science
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Health Care Quality, Access, and Evaluation
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Eukaryota
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Anthropology, Education, Sociology and Social Phenomena
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Organisms
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Natural Science Disciplines
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Health Care
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Disciplines and Occupations
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Genetics
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Sciences - General
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Law, General & Comparative
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Law, Politics & Government
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Physical Sciences & Mathematics
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Biology
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Health & Biological Sciences
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Chimeras (Botany)
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Transgenic animals.
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Moral and ethical aspects.
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Chimeras, Plant
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Plant chimaeras
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Plant chimeras
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Research ethics
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Chimeras and Hybrids in Comparative European and International Research (project)
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Law.
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Ethics.
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Transgenic organisms.
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Law
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Private international law.
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Conflict of laws.
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International law.
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Comparative law.
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Medical laws and legislation.
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Private International Law, International & Foreign Law, Comparative Law.
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Medical Law.
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Theories of Law, Philosophy of Law, Legal History.
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Transgenics.
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Philosophy.
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Law, Medical
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Medical personnel
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Medical registration and examination
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Medicine
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Physicians
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Surgeons
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Medical policy
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Medical jurisprudence
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Comparative jurisprudence
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Comparative legislation
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Jurisprudence, Comparative
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Law, Comparative
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Legislation, Comparative
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Law of nations
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Nations, Law of
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Public international law
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Choice of law
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Conflict of laws
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Intermunicipal law
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International law, Private
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International private law
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Private international law
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Legal polycentricity
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Jurisprudence
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Genetically engineered organisms
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Genetically modified organisms
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GEOs (Genetically engineered organisms)
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GMOs (Genetically modified organisms)
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Deontology
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Ethics, Primitive
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Ethology
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Moral philosophy
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Morality
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Morals
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Philosophy, Moral
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Science, Moral
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Philosophy
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Values
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Acts, Legislative
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Enactments, Legislative
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Laws (Statutes)
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Legislative acts
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Legislative enactments
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Legislation
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Legal status, laws, etc.
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Law and legislation
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Civil law
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Transgenic organisms
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Animal genetic engineering
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Botany
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Chimerism
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Plant genetics
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Public health laws.
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Private International Law, International & Foreign Law, Comparative Law .
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Communicable diseases
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Public health
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Medical laws and legislation
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Law—Philosophy.
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Europäische Union
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Mensch
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Chimäre
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As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
Corn --- Transgenic plants --- Laboratory Manuals --- Zea mays --- Plants, Genetically Modified --- Genetics --- Cereals --- Plants --- Organisms, Genetically Modified --- Publication Formats --- Animal Feed --- Biology --- Poaceae --- Angiosperms --- Organism Forms --- Publication Characteristics --- Eukaryota --- Food --- Crops, Agricultural --- Biological Science Disciplines --- Organisms --- Natural Science Disciplines --- Embryophyta --- Food and Beverages --- Technology, Industry, Agriculture --- Streptophyta --- Disciplines and Occupations --- Viridiplantae --- Plant Sciences --- Agriculture --- Earth & Environmental Sciences --- Genetic engineering --- Transgenic plants. --- Genetic engineering. --- genetics --- Beverages and Food --- Land Plants --- Land Plant --- Plant, Land --- Plants, Land --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Agricultural Crops --- Agricultural Crop --- Nutrients --- Foods --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Angiospermae --- Liliopsida --- Magnoliopsida --- Angiosperm --- Liliopsidas --- Magnoliopsidas --- Grass --- Imperata cylindrica --- Alopecurus --- Arundo --- Gramineae --- Grasses --- Imperata --- Fodder --- Animal Feeds --- Feed, Animal --- Feeds, Animal --- Fodders --- GE crops (Genetically engineered crops) --- GE plants (Genetically engineered plants) --- Genetically engineered crops --- Genetically engineered plants --- Genetically modified crops --- Genetically modified plants --- GM crops (Genetically modified crops) --- GM plants (Genetically modified plants) --- Novel crops --- Transgenic crops --- GMO Organisms --- Genetically Engineered Organisms --- Genetically Modified Organisms --- Organisms, Transgenic --- Transgenic Organisms --- Engineered Organism, Genetically --- Engineered Organisms, Genetically --- GMO Organism --- Genetically Engineered Organism --- Genetically Modified Organism --- Modified Organism, Genetically --- Modified Organisms, Genetically --- Organism, GMO --- Organism, Genetically Engineered --- Organism, Genetically Modified --- Organism, Transgenic --- Organisms, GMO --- Organisms, Genetically Engineered --- Transgenic Organism --- Plant --- Cereal Grain --- Cereal --- Cereal Grains --- Edible Grains --- Grain, Cereal --- Grain, Edible --- Grains, Cereal --- Grains, Edible --- GMO Plants --- Genetically Engineered Plants --- Genetically Modified Plants --- Plants, Transgenic --- Transgenic Plants --- Engineered Plant, Genetically --- Engineered Plants, Genetically --- GMO Plant --- Genetically Engineered Plant --- Genetically Modified Plant --- Modified Plant, Genetically --- Modified Plants, Genetically --- Plant, GMO --- Plant, Genetically Engineered --- Plant, Genetically Modified --- Plant, Transgenic --- Plants, GMO --- Plants, Genetically Engineered --- Transgenic Plant --- Indian Corn --- Maize --- Teosinte --- Zea --- Corn, Indian --- Green Plants --- Green Plant --- Plant, Green --- Plants, Green --- Agriculture. --- Botany. --- Plant genetics. --- Transgenic organisms. --- Plant Sciences. --- Plant Genetics and Genomics. --- Transgenics. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Natural history --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Floristic botany
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The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular Biology™ series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.
Transgenic plants --- Wheat --- Barley --- Oats --- Avena sativa --- Hordeum --- Laboratory Manuals --- Triticum --- Plants, Genetically Modified --- Genetic Engineering --- Methods --- Genetics --- Cereals --- Animal Feed --- Publication Formats --- Biology --- Poaceae --- Investigative Techniques --- Organisms, Genetically Modified --- Plants --- Genetic Techniques --- Publication Characteristics --- Food --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Organism Forms --- Crops, Agricultural --- Angiosperms --- Eukaryota --- Embryophyta --- Food and Beverages --- Organisms --- Natural Science Disciplines --- Disciplines and Occupations --- Technology, Industry, Agriculture --- Streptophyta --- Viridiplantae --- Plant Sciences --- Agriculture --- Earth & Environmental Sciences --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Beverages and Food --- Land Plants --- Land Plant --- Plant, Land --- Plants, Land --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Angiospermae --- Liliopsida --- Magnoliopsida --- Angiosperm --- Liliopsidas --- Magnoliopsidas --- Agricultural Crops --- Agricultural Crop --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Nutrients --- Foods --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Plant --- GMO Organisms --- Genetically Engineered Organisms --- Genetically Modified Organisms --- Organisms, Transgenic --- Transgenic Organisms --- Engineered Organism, Genetically --- Engineered Organisms, Genetically --- GMO Organism --- Genetically Engineered Organism --- Genetically Modified Organism --- Modified Organism, Genetically --- Modified Organisms, Genetically --- Organism, GMO --- Organism, Genetically Engineered --- Organism, Genetically Modified --- Organism, Transgenic --- Organisms, GMO --- Organisms, Genetically Engineered --- Transgenic Organism --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Grass --- Imperata cylindrica --- Alopecurus --- Arundo --- Gramineae --- Grasses --- Imperata --- Fodder --- Animal Feeds --- Feed, Animal --- Feeds, Animal --- Fodders --- Cereal Grain --- Cereal --- Cereal Grains --- Edible Grains --- Grain, Cereal --- Grain, Edible --- Grains, Cereal --- Grains, Edible --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- GMO Plants --- Genetically Engineered Plants --- Genetically Modified Plants --- Plants, Transgenic --- Transgenic Plants --- Engineered Plant, Genetically --- Engineered Plants, Genetically --- GMO Plant --- Genetically Engineered Plant --- Genetically Modified Plant --- Modified Plant, Genetically --- Modified Plants, Genetically --- Plant, GMO --- Plant, Genetically Engineered --- Plant, Genetically Modified --- Plant, Transgenic --- Plants, GMO --- Plants, Genetically Engineered --- Transgenic Plant --- Durum Wheat --- Triticum aestivum --- Triticum durum --- Triticum spelta --- Triticum turgidum --- Triticum turgidum subsp. durum --- Triticum vulgare --- Durum Wheats --- Wheat, Durum --- Hordeum vulgare --- Avena --- Common oat --- Oat, Common --- GE crops (Genetically engineered crops) --- GE plants (Genetically engineered plants) --- Genetically engineered crops --- Genetically engineered plants --- Genetically modified crops --- Genetically modified plants --- GM crops (Genetically modified crops) --- GM plants (Genetically modified plants) --- Novel crops --- Transgenic crops --- methods --- Green Plants --- Green Plant --- Plant, Green --- Plants, Green --- Cultivated Oat --- Avenas --- Cultivated Oats --- Oat --- Oat, Cultivated --- Oats, Cultivated --- Chemistry. --- Biotechnology. --- Genetic engineering. --- Transgenic organisms. --- Genetic Engineering. --- Food Science. --- Transgenics. --- Food science. --- Science --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Genetic engineering --- Designed genetic change --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Chemical engineering --- Food technology
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