Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology™ series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.

Entomology. --- Microbial genetics. --- Microbial genomics. --- Proteomics. --- Mycology. --- Microbial Genetics and Genomics. --- Insects --- Zoology --- Genomics --- Microbial genetics --- Microorganisms --- Genetics --- Microbiology --- Molecular biology --- Proteins

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The major difficulty many microbiologists face is simply that of too much information. As a result of sequencing technologies becoming so economical, there is a very real and pressing need for high-throughput computational methods to compare hundreds and thousands of bacterial genomes. This accessible text/reference provides a coherent set of tools and a methodological framework for comparing raw DNA sequences and fully annotated genome sequences, then using these to build up and test models about groups of interacting organisms within an environment or ecological niche. Easy-to-follow, this introductory textbook is built around teaching computational / bioinformatics methods for comparison of microbial genomes, and includes detailed examples of how to compare them at the level of DNA, RNA, and protein, in terms of structural and functional analysis. Topics and Features: • Contains five introductory chapters each representing a specific scientific field, to bring all readers up to the same basic level • Familiarizes readers with genome sequences, RNA sequences (transcriptomics), proteomics and regulation of gene expression • Describes basic methods to compare genomes and visualize the results for easy interpretation • Discusses microbial communities, providing a framework for analysing and comparing individual genomes or raw DNA derived from complete ecosystems • Introduces various atlases, building up to the Genome Atlas • Offers numerous helpful examples throughout • Focuses on the use and interpretation of publicly available Web tools • Provides supplemental resources, such as Web links, at http://comparativemicrobial.com Developed from a set of lectures for a course in Comparative Microbial Genomics taught since 2001, this wide-ranging foundational textbook is aimed at advanced undergraduate and graduate students in Bioinformatics and Microbiology. The authors are from diverse backgrounds complementing the interdisciplinary nature of the topic and consequently have developed a common scientific language. Readers will find this text an invaluable reference for computational and bioinformatics tools.

Bioinformatics. --- Electronic books. -- local. --- Microbial genomics -- Data processing. --- Microbial genomics --- Bioinformatics --- Genetics, Microbial --- Genome, Bacterial --- Computational Biology --- Biology --- Genetics --- Genome --- Biological Science Disciplines --- Genetic Structures --- Genetic Phenomena --- Natural Science Disciplines --- Disciplines and Occupations --- Phenomena and Processes --- Biology - General --- Health & Biological Sciences --- Data processing --- Data processing. --- Bio-informatics --- Biological informatics --- Life sciences. --- Human genetics. --- Computer science. --- Computers. --- Microbiology. --- Microbial genetics. --- Microbial genomics. --- Life Sciences. --- Computer Science, general. --- Computational Biology/Bioinformatics. --- Microbial Genetics and Genomics. --- Computing Methodologies. --- Human Genetics. --- Information science --- Computational biology --- Systems biology --- Genomics --- Microbial genetics --- Artificial intelligence. --- Artificial Intelligence. --- Microbial biology --- Microorganisms --- Heredity, Human --- Human biology --- Physical anthropology --- AI (Artificial intelligence) --- Artificial thinking --- Electronic brains --- Intellectronics --- Intelligence, Artificial --- Intelligent machines --- Machine intelligence --- Thinking, Artificial --- Bionics --- Cognitive science --- Digital computer simulation --- Electronic data processing --- Logic machines --- Machine theory --- Self-organizing systems --- Simulation methods --- Fifth generation computers --- Neural computers --- Microbiology --- Informatics --- Science

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Achievements and progress in genome mapping and the genomics of microbes supersede by far those for higher plants and animals, in part due to their enormous economic implication but also smaller genome size. In the post-genomic era, whole genome sequences of animal-associated microbes are providing clues to depicting the genetic basis of the complex host-pathogen relationships and the evolution of parasitism; and to improving methods of controlling pathogens. This volume focuses on a globally important group of intracellular prokaryotic pathogens which affect livestock animals. These include Brucella, Mycobacterium, Anaplasma and Ehrlichia, as well as the protozoan pathogens Cryptosporidium and Theileria, for which genome sequence data is available. Insights from comparative genomics of the microbes described provide clues to the adaptation involved in host-microbe interactions, as well as resources potentially useful for application in future research and product development.

Intracellular pathogens. --- Microbial ecology. --- Microbial genomics. --- Microbial genomics --- Intracellular pathogens --- Chromosome Mapping --- Genomics --- Methods --- Genetics --- Gram-Negative Bacteria --- Animals, Domestic --- Investigative Techniques --- Biology --- Computational Biology --- Animal Population Groups --- Genetic Techniques --- Bacteria --- Organisms --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animals --- Natural Science Disciplines --- Eukaryota --- Disciplines and Occupations --- Health & Biological Sciences --- Gene mapping. --- Microbial genetics. --- Microorganisms --- Chromosome mapping --- Genetic mapping --- Genome mapping --- Linkage mapping (Genetics) --- Mapping, Gene --- Life sciences. --- Veterinary medicine. --- Animal physiology. --- Life Sciences. --- Microbial Genetics and Genomics. --- Animal Physiology. --- Veterinary Medicine. --- Microbiology --- Technique --- Veterinary Medicine/Veterinary Science. --- Farriery --- Large animal medicine --- Large animal veterinary medicine --- Livestock medicine --- Veterinary science --- Medicine --- Animal health --- Domestic animals --- Livestock --- Animal physiology --- Anatomy --- Microbial genetics --- Diseases --- Losses --- Physiology

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. Coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. Coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. Coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.

Escherichia coli. --- Systems biology. --- Escherichia coli --- Systems biology --- Systems Biology --- Escherichia --- Computational Biology --- Biology --- Enterobacteriaceae --- Biological Science Disciplines --- Gram-Negative Facultatively Anaerobic Rods --- Gammaproteobacteria --- Natural Science Disciplines --- Proteobacteria --- Gram-Negative Bacteria --- Bacteria --- Disciplines and Occupations --- Organisms --- Biochemistry --- Biology - General --- Microbiology & Immunology --- Chemistry --- Health & Biological Sciences --- Physical Sciences & Mathematics --- Biochemistry. --- Biotechnology. --- Life sciences. --- Microbial genetics. --- Microorganisms --- Biosciences --- Sciences, Life --- Biological chemistry --- Chemical composition of organisms --- Physiological chemistry --- E. coli (Bacterium) --- Genetics --- Composition --- Microbiology. --- Microbial genomics. --- Life Sciences. --- Biochemistry, general. --- Microbial Genetics and Genomics. --- Applied Microbiology. --- Microbiology --- Science --- Chemical engineering --- Genetic engineering --- Medical sciences --- Genomics --- Microbial genetics --- Microbial biology

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Megaplasmids are extrachromosomal genetic elements in the size range of 100 kb and larger. They are found in physiologically and phylogenetically diverse groups of bacteria and archaea. By definition, megaplasmids are not essential for the viability of their hosts under all growth conditions, but paradoxically many megaplasmids carry the genetic information for the defining and characteristic traits of the organism in which they reside. Microbial Megaplasmids reviews our knowledge of the extensively studied representatives, such as the catabolic plasmids of the pseudomonads, the rhizobial Sym plasmids, the Ti plasmids of the genus Agrobacterium and the giant enterobacterial virulence plasmids. It also presents snapshots of more recently discovered megaplasmids. The contribution of megaplasmids to the biology of their hosts is described, highlighting the interactions between megaplasmid and chromosomal genes.

Agriculture. --- Bacterial chromosomes. --- Bacteriology. --- Chromosomes, Bacterial. --- DNA Transposable Elements --genetics. --- DNA, Bacterial. --- Genome, Bacterial. --- Microbial genetics. --- Microbiology. --- Plant diseases. --- Plasmids --genetics. --- Plasmids. --- Microorganisms --- Paragenes --- Genetics --- Life sciences. --- Medical microbiology. --- Microbial genomics. --- Plant pathology. --- Life Sciences. --- Plant Pathology. --- Medical Microbiology. --- Microbial Genetics and Genomics. --- Applied Microbiology. --- Microbiology --- Botany --- Communicable diseases in plants --- Crop diseases --- Crops --- Diseases of plants --- Microbial diseases in plants --- Pathological botany --- Pathology, Vegetable --- Phytopathology --- Plant pathology --- Plants --- Vegetable pathology --- Agricultural pests --- Crop losses --- Diseased plants --- Phytopathogenic microorganisms --- Plant pathologists --- Plant quarantine --- Microbial biology --- Biology --- Genomics --- Microbial genetics --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Biosciences --- Sciences, Life --- Science --- Pathology --- Diseases and pests --- Diseases --- Wounds and injuries --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA