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The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Biological microscopy

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Acqui 2006 --- Microscopy, Electron. --- Physics. --- Electron microscopy

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In der vorliegenden Arbeit wurde ein elektrochemisches Raster-Sonden-Mikroskop dazu benutzt, gezielt Nanostrukturen herzustellen und zu charakterisieren. Das elektrochemische Umfeld bietet gegenüber den sonst angewandten Techniken den Vorteil, dass die Über- und Untersättigung an den beiden Arbeitselektroden frei eingestellt werden kann. Diese Methode erlaubt es lokal Nanostrukturen definierter Größe herzustellen. Mittels einer selbst aufgebauten UHV-Apparatur konnten Präparationstechniken zur reproduzierbaren Herstellung von geometrisch hoch definierten STM-Spitzen für nahezu jedes Metall entwickelt werden. Derart qualitativ hochwertige STM-Spitzen ermöglichten erstmals einen Strommessbereich von 10-6 bis10-12 Ampere abzudecken. Die entwickelten Instrumente konnten zur Untersuchung der Systeme Co bzw. Ni auf Au(111)-Substraten eingesetzt werden. Das Verfahren zur Erzeugung magnetischer Nanostrukturen wurde optimiert und erste spektroskopische Messungen an der Fest-/ Flüssiggrenzfläche konnten durchgeführt werden.

Scanning tunneling microscopy. --- Scanning probe microscopy.

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Microscopy --- #KVHA:Microscopie. Woordenboeken. Engels --- #KVHA:Natuurwetenschappen. Woordenboeken. Engels --- Microscopy. --- Microscopies --- Microscopen --- Microscopie --- woordenboeken --- woordenboeken. --- Woordenboeken. --- Compound Microscopy --- Hand-Held Microscopy --- Light Microscopy --- Optical Microscopy --- Simple Microscopy --- Hand Held Microscopy --- Microscopy, Compound --- Microscopy, Hand-Held --- Microscopy, Light --- Microscopy, Optical --- Microscopy, Simple --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Microscopy - Dictionaries

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Image analysis. --- Microscopy.