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Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach.

Cellular signal transduction --- G proteins --- Cell receptors --- GTP-Binding Proteins --- Signal Transduction --- analysis --- physiology --- Cell membrane receptors --- Cell surface receptors --- Receptors, Cell --- Binding sites (Biochemistry) --- Cell membranes --- Proteins --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cellular signal transduction - Laboratory manuals --- G proteins - Laboratory manuals --- Cell receptors - Laboratory manuals --- GTP-Binding Proteins - analysis - laboratory manuals --- Signal Transduction - physiology - Laboratory Manuals

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In front of you is the finished product of your work, the text of your contributions to the 2003 Dayton International Symposium on Cell Volume and Signal Transduction. As we all recall, this symposium brought together the Doyens of Cellular and Molecular Physiology as well as aspiring young investigators and students in this field. It became a memorable event in an illustrious series of International Symposia on Cell Volume and Signaling. This series, started by Professors Vladimir Strbák, Florian Lang and Monte Greer in Smolenice, Slovakia in 1997 and continued by Professors Rolf Kinne, Florian Lang and Frank Wehner in Berlin in 2000, is projected for 2005 in Copenhagen to be hosted by our colleague, Professor Else Hoffmann and her team. We dearly miss Monte Greer to whom this symposium was dedicated and addressed so eloquently by Vladimir Strbák in his Dedication to Monte. Monte and I became friends in Smolenice and had begun to discuss the 2003 meeting only a few days before his tragic accident in 2002. There are others who were not with us, and we missed them, too. We would not have been able to succeed in this event without the unflagging support of our higher administration at Wright State University, the NIDDKD of the National Institute of Health, and the Fuji Medical System (see Acknowledgments).

Cells -- Morphology -- Congresses. --- Cellular signal transduction -- Congresses. --- Cellular signal transduction --- Cells --- Symporters --- Cell Physiological Phenomena --- Cell Death --- Membrane Glycoproteins --- Peptides --- Proteins --- Membrane Transport Proteins --- Cell Physiological Processes --- Biochemical Processes --- Cytoplasmic Structures --- Biological Factors --- Amino Acids, Peptides, and Proteins --- Membrane Proteins --- Phenomena and Processes --- Biochemical Phenomena --- Cytoplasm --- Chemical Processes --- Ion Pumps --- Chemicals and Drugs --- Carrier Proteins --- Chemical Phenomena --- Intracellular Space --- Cellular Structures --- Anatomy --- Ion Channels --- Intercellular Signaling Peptides and Proteins --- Signal Transduction --- Cytoskeleton --- Sodium-Potassium-Chloride Symporters --- Cell Size --- Apoptosis --- Human Anatomy & Physiology --- Health & Biological Sciences --- Neuroscience --- Animal Biochemistry --- Morphology --- Life sciences. --- Human physiology. --- Molecular biology. --- Neurosciences. --- Cell biology. --- Neurobiology. --- Life Sciences. --- Cell Biology. --- Human Physiology. --- Molecular Medicine. --- Life Sciences, general. --- Neurosciences --- Cell biology --- Cellular biology --- Biology --- Cytologists --- Neural sciences --- Neurological sciences --- Medical sciences --- Nervous system --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Human biology --- Physiology --- Human body --- Biosciences --- Sciences, Life --- Science --- Organisms --- Cytology --- Cytology. --- Medicine. --- Clinical sciences --- Medical profession --- Life sciences --- Pathology --- Physicians

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G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cellular signal transduction --- G proteins --- 57.088 --- 577.112 --- 577.38 --- 577.38 Theoretical biophysics. Biophysics of control processes. Biophysics of communication --- Theoretical biophysics. Biophysics of control processes. Biophysics of communication --- 577.112 Proteins --- Proteins --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology