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Plants depend on physiological mechanisms to combat adverse environmental conditions, such as pathogen attack, wounding, drought, cold, freezing, salt, UV, intense light, heavy metals and SO2. Many of these cause excess production of active oxygen species in plant cells. Plants have evolved complex defense systems against such oxidative stress, and biologists are now studying these mechanisms in-depth. Oxidative Stress in Plants explores how various biotic and abiotic environmental stress conditions produce oxygen radicals. It explains the biochemistry and molecular biology of both non-enzymatic (vitamin C, glutathione) and enzymatic systems which eliminate active oxygen species.
Oxidation, Physiological. --- Plant defenses. --- Oxidation, Physiological --- Plant defenses --- Defense mechanisms, Plant --- Defenses, Plant --- Mechanisms, Plant defense --- Plant defense mechanisms --- Plant self-protection --- Plants --- Self-defense in plants --- Self-protection in plants --- Plant ecology --- Dangerous plants --- Poisonous plants --- Biological oxidation --- Oxidation, Biological --- Physiological oxidation --- Biochemistry --- Defenses
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Oxidative stress has been linked to a variety of medical problems, including cardiovascular disease, cancer, arthritis, diabetes, and aging. Drawing on the growing volume of research into the impact of oxidative stress on pathophysiological mechanisms, Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the perturbations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format by accomplished investigators from prestigious universities, institutes, and laboratories around the world, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltammetry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols (Humana, 2002), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays. Cutting-edge and highly practical, Oxidative Stress Biomarkers and Antioxidant Protocols provides many powerful new tools for detecting oxidative stress and for investigating the possibilities for therapeutic intervention monitoring in a variety of serious diseases.
Molecular biology --- Antioxidants -- Physiological effect -- Laboratory manuals. --- Electronic books. -- local. --- Free radicals (Chemistry) -- Pathophysiology -- Laboratory manuals. --- Oxidation, Physiological -- Laboratory manuals. --- Stress (Physiology) -- Research -- Laboratory manuals. --- Free radicals (Chemistry) --- Antioxidants --- Oxidative stress --- Free Radicals --- Oxidative Stress --- Protective Agents --- Molecular Mechanisms of Pharmacological Action --- Stress, Physiological --- Inorganic Chemicals --- Organic Chemicals --- Metabolism --- Chemicals and Drugs --- Metabolic Phenomena --- Physiological Effects of Drugs --- Pharmacologic Actions --- Physiological Processes --- Specialty Uses of Chemicals --- Chemical Actions and Uses --- Physiological Phenomena --- Phenomena and Processes --- Biology - General --- Pathology --- Biochemistry --- Biology --- Chemistry --- Medicine --- Physical Sciences & Mathematics --- Health & Biological Sciences --- Pathophysiology --- Physiological effect --- Research --- Oxidation, Physiological --- Stress (Physiology) --- Physiological stress --- Tension (Physiology) --- Adaptation (Biology) --- Biological oxidation --- Oxidation, Biological --- Physiological oxidation --- Chemical inhibitors --- Radicals (Chemistry) --- Free radical reactions --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition
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