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2002 (14)

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Book
Acacia spp.
Authors: --- --- --- --- --- et al.
ISBN: 9290435240 Year: 2002 Publisher: Rome : IPGRI,

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Book
Pinus spp.
Authors: --- --- --- ---
ISBN: 9290435259 Year: 2002 Publisher: Rome : IPGRI,

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Dissertation
Study of the implication of exo-beta-1, 3-glucanase encoding genes in the biocontrol activity of Pichia anomala (strain K) against Botrytis cinerea on post-harvest apples.
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Year: 2002

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The yeast P. anomala strain K protects post-harvest apples against Botrytis cinerea. Two genes encoding exo-beta-1, 3-glucanases, PaEXG1 and PaEXG2 have been isolated from strain K genome thanks to conserved regions in fungal exo-beta-1, 3-glucanases and to the sequencing of PaEXG2, an exo-beta-1, 3-glucanase from strain K culture fluids. PaEXG1 potentially encodes a 498 amino acids long protein, with a calculated molecular weight of 58 kDa, an estimated pI of 4.79 and 8 potential sites for N-linked glycosylation. PaEXG2 has a coding capacity for an acidic protein of 427 amino acids with a predicted molecular weight of 49 kDa, a calculated pI of 4.70 and one potential N-glycosylation site. Haploid uracil auxotrophic mutants derived from strain K showed an inferior biocontrol effect and colonization of apple wounded sites compared to the prototrophic strain. Antagonism and colonization were recovered after restoration of the prototrophy by transformation with the URA3 gene encoding orotidine monophosphate decarboxylase. PaEXG1 and PaEXG2 genes were separately disrupted by the insertion of the URA3 marker gene in their ORFs. Integrative transformation was shown to be mostly ectopic in strain K descendants (only 4 % of integration by homologous recombination). Disruption of PaEXG1 or PaEXG2 had no visible effect on in vitro growth with B. cinerea cell walls preparation as the sole carbon source or in apple wounds. PaEXG1 disruption had no effect on the in vitro or in situ extracellular exo-beta-1, 3-glucanases activity production while PaEXG2 disruption abolished all detectable activity in vitro and in vivo. Disruption of PaEXG1 or PaEXG2 did not affect the biocontrol towards B. cinerea on wounded apples, showing that the products of those genes are dispensable for protective activity (in singly disrupted mutants).


Dissertation
Cartographie de marqueurs RFLP de froment et mise en évidence de QTL de résistance à l'oïdium.
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Year: 2002

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Wheat RFLP markers were selected for providing a readable hybridization pattern and characterized for their polymorphism information content. We report the mapping of 160 loci, corresponding to 132 markers, on the genetic map of a wheat reference population. This map resulting from joined works of different labs, constitutes an essential tool for further molecular marker assisted wheat breeding. Molecular markers were used to study the genetic basis of the quantitative resistance to powdery mildew of the wheat line RE714. Two doubled haploid populations from the crosses between RE714 and the susceptible parents Festin and Hardi were used. Using RFLP and microsatellites markers, genetic maps were developed for both populations ; line resistance was observed in field tests over different environments. Considering both populations and all the environments tested, the segregation data from molecular markers and resistance allowed us to detect 9 QTL (Quantitative Trait Loci) involved in the quantitative resistance of RE714. Among them, we found residual effects of the race-specific resistance genes Pm4b and MIRE. Two major QTL were very stable since they were detected across the two populations and all environments tested. Comparing the two populations, we show differences in the QTL detected according to the susceptible parent used and that the genetic background of Hardi leads to a lack of residual effect at the Pm4b gene.


Book
Evaluation globale de la résistance des bananiers à la fusariose, aux maladies foliaires causées par les Mycosphaerella spp. et aux nématodes : Evaluation approfondie.
Authors: --- --- --- ---
ISBN: 2910810534 Year: 2002 Publisher: Montpellier : INIBAP,

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Dissertation
Mechanisms of action in biological control of Fusarium oxysporum f. sp. lycopersici in tomato using rhizobacteria.
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Year: 2002 Publisher: Bonn : Rheinischen Friedrich-Wilhelms-Universität,


Dissertation
Basal and induced systemic resistance to Botrytis cinerea in tomato.
Author:
Year: 2002 Publisher: Gent : Universiteit Gent. Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen,


Dissertation
Diversity and ecology of the potato : The use of spatial analysis in crop science.
Author:
Year: 2002 Publisher: Wageningen : Wageningen University,


Dissertation
Use of the tomato Cf9-Avr9 system to engineer disease resistance in oilseed rape.
Author:
Year: 2002 Publisher: Gent : Universiteit Gent. Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen,


Book
Proceedings of the IInd international symposium on cucurbits : Tsukuba, Japan, 28 September-1 October, 2001.
Author:
ISBN: 9066057769 Year: 2002 Publisher: Leuven : International Society for Horticultural Science,

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