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Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.

Nucleotide sequence --- Génome humain --- Laboratory manuals --- Séquençage --- Manuels de laboratoire --- DNA. --- Electronic books. -- local. --- Nucleotide sequence -- Laboratory manuals. --- Sequence Analysis --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Sequence Analysis, DNA --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Génome humain --- Séquençage --- Deoxyribonucleic acid --- Desoxyribonucleic acid --- Thymonucleic acid --- TNA (Nucleic acid) --- Deoxyribose --- Nucleic acids --- Genes --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Nucleotides --- Sequence alignment (Bioinformatics) --- Analysis --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells