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In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.
Electronic books. -- local. --- Killer cells -- Immunology. --- Killer cells -- Laboratory manuals. --- Killer cells --- Killer Cells, Natural --- Cytotoxicity Tests, Immunologic --- Methods --- Lymphocytes --- Investigative Techniques --- Toxicity Tests --- Immunologic Tests --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Laboratory Techniques and Procedures --- Immunologic Techniques --- Leukocytes, Mononuclear --- Leukocytes --- Diagnosis --- Immune System --- Blood Cells --- Cells --- Hemic and Immune Systems --- Blood --- Anatomy --- Microbiology & Immunology --- Biology --- Health & Biological Sciences --- immunology. --- methods. --- Immunology. --- K cells --- Natural killer cells --- NK cells --- Immunocompetent cells --- Cell-mediated cytotoxicity --- Immunobiology --- Life sciences --- Serology
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Endocrine and immune parameters, namely glucocorticoid receptors (GcR) and IFN-gamma production in peripheral blood mononuclear cells (PBMC), and corticosterone in plasma, were studied in groups of four male rabbits observed in seminatural conditions in relation to agonistic behaviour and to seasonal variations. These parameters were selected on the basis of the findings that the presence of GcR in PBMC gives an indication of the in vivo biological effects of glucocorticoids and that PBMC cytokine production and plasma corticosterone are related to social behaviour. The frequency of active and passive behaviours was used to rank the animals. Seasonal variations were present for agonistic behaviours (Attack, Follow, Chase) and For plasma corticosterone, which were significantly higher in winter. IFN-gamma production in PBMC was increased after social interactions in both seasons, while plasma corticosterone was increased only in winter: GcR capacity in PBMC was decreased after social interactions. The results indicate that social and physical environmental factors are correlated with immune-endocrine functions
Agonistic behaviour,seasonal variation,corticosterone,glucocorticoid receptors,ifn-gamma production,rabbit. --- Agonistic behaviour. --- Agonistic. --- Animal. --- Animals. --- Behavior. --- Behaviour. --- Blood mononuclear-cells. --- Blood. --- Corticosterone. --- Dominance. --- Endocrine. --- Frequency. --- Function. --- Glucocorticoid receptors. --- Glucocorticoid. --- Glucocorticoids. --- Group. --- Hippocampal electrical-activity. --- Immune. --- Interaction. --- Interactions. --- Invivo. --- Male. --- Parameters. --- Physical. --- Pituitary. --- Plasma corticosterone. --- Plasma-corticosterone. --- Plasma. --- Production. --- Rabbit. --- Rabbits. --- Rank. --- Receptor. --- Receptors. --- Release. --- Responses. --- Season. --- Seasonal variation. --- Social behaviour. --- Social interaction. --- Social interactions. --- Social-interaction. --- Social. --- Stress. --- Variation.
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