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In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results.Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today.
Transcription Factors --- Sequence Analysis, DNA --- Transfection --- Transcription factors --- Facteurs de transcription --- analysis --- methods --- Laboratory manuals --- Manuels de laboratoire --- Laboratory manuals. --- analysis. --- methods. --- Sequence analysis, dna --- Methods. --- Analysis. --- Proteins. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Genetic transcription factors --- Proteins --- Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology
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Molecular biology --- Genomes --- Gene mapping --- Genomics --- Genetics --- Chromosome Mapping --- Base Sequence --- Sequence Analysis, DNA --- Genetic Techniques --- Génomes --- Cartes chromosomiques --- Génomique --- Génétique --- Periodicals --- Technique --- Périodiques --- Genomics. --- Base Sequence. --- Chromosome Mapping. --- Genetic Techniques. --- Sequence Analysis, DNA. --- Gene mapping. --- Genomes. --- Genetics & Genomics. --- Analysis, DNA Sequence --- DNA Sequence Determination --- DNA Sequence Determinations --- DNA Sequencing --- Determination, DNA Sequence --- Determinations, DNA Sequence --- Sequence Determinations, DNA --- DNA Sequence Analysis --- Sequence Determination, DNA --- Analyses, DNA Sequence --- DNA Sequence Analyses --- Sequence Analyses, DNA --- Sequencing, DNA --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genome Mapping --- Gene Mapping --- Linkage Mapping --- Chromosome Mappings --- Gene Mappings --- Genome Mappings --- Linkage Mappings --- Mapping, Chromosome --- Mapping, Gene --- Mapping, Genome --- Mapping, Linkage --- Mappings, Chromosome --- Mappings, Gene --- Mappings, Genome --- Mappings, Linkage --- DNA Sequences --- DNA Sequence --- Nucleotide Sequence --- RNA Sequence --- Base Sequences --- Nucleotide Sequences --- RNA Sequences --- Sequence, Base --- Sequence, DNA --- Sequence, Nucleotide --- Sequence, RNA --- Sequences, Base --- Sequences, DNA --- Sequences, Nucleotide --- Sequences, RNA --- Chromosome mapping --- Genetic mapping --- Genome mapping --- Linkage mapping (Genetics) --- methods --- genomics --- genome-scale analysis --- functional genomics --- proteomics --- Haploidy --- Molecular Sequence Annotation --- Genetic Counseling --- Genetic Phenomena --- Cytogenetics --- Genetic Markers --- Cytogenetic Analysis --- Gene Order --- Synteny --- Genes --- Nucleotides --- Human Genome Project --- Genome --- Comparative Genomics --- Comparative Genomic --- Genomic, Comparative --- Genomics, Comparative --- Biotechnology --- moleculaire biologie --- biotechnologie
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