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This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. Step-by-step protocols and practical notes are provided. The distinguishing feature of this manual compared to others is the detailed explanation of the theoretical mechanisms of each step and the discussion of the importance of the techniques. In addition, each protocol includes an indication of the time and expense involved in its application. This knowledge will enable the users to design their own modifications or to adapt the method to different systems. Dr. Surzycki has been teaching undergraduate courses and leading workshops in introductory Molecular Biology for many years, during which time he has extensively modified and refined the techniques he has described in this manual. Keywords: DNA - DNA Isolierung - DNA sequencing - Nucleic acids - Nukleinsäuren - PCR - Polymerasekettenreaktion - RNA - Seuqenzierung - Southern Blot - biology - cloning - hybridization - molecular biology - preparation
General biochemistry --- polymers --- biotechnologie --- biochemie --- Biotechnology --- Molecular biology --- moleculaire biologie --- polymeren --- Macromolecules --- Molecular genetics --- Biochemistry --- Genetics --- Chemistry --- Biology --- Biological Science Disciplines --- Natural Science Disciplines --- Disciplines and Occupations --- Molecular Biology --- Health & Biological Sciences --- Biophysics --- klonen --- electrophoresis --- PCR (polymerase chain reaction) --- Biology—Technique. --- Biochemistry. --- Medicine. --- Biotechnology. --- Plant genetics. --- Biological Techniques. --- Biochemistry, general. --- Biomedicine, general. --- Plant Genetics and Genomics. --- Plants --- Chemical engineering --- Genetic engineering --- Health Workforce --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition --- Molecular biology - Laboratory manuals. --- Molecular genetics - Laboratory manuals.
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The central theme of Green Plants, first published in 2000, is the astonishing diversity of forms found in the plant kingdom, from the simplicity of prokaryotic algae to the myriad complexities of flowering plants. The book is arranged according to generally accepted classification schemes, beginning with algae (prokaryotic and eukaryotic) and moving through mosses, liverworts, fern allies, ferns and gymnosperms to flowering plants. Copiously illustrated throughout, it provides a concise account of all algae and land plants, with information on topics from cellular structure to life cycles and reproduction. The authors maintain a refreshingly cautious approach in discussions of possible phylogenetic relationships and include newly emerging information on features of plants known only as fossils. This edition has been completely updated to reflect current views on the origin of the major groups of plants, providing a resource for students of botany, and for researchers needing a comprehensive reference to the plant kingdom.
Botany. --- Plants --- Evolution. --- Variation. --- Botany --- Plant species --- Plant variation --- Plant evolution --- Botanical classification --- Botanical systematics --- Botanical taxonomy --- Classification --- Plant biosystematics --- Plant classification --- Plant systematics --- Plant taxonomy --- Systematic botany --- Systematics (Botany) --- Taxonomy, Plant --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Variation --- Plant diversity --- Plant genetics --- Variation (Biology) --- Evolution (Biology) --- Plant taxonomists --- Biology --- Natural history --- Phylogeny --- Floristic botany --- Evolution --- Botan. systematics, taxonomy, nomencl. --- Plant genetics. Plant evolution --- Phanerogams
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Economic and applied botany --- Plant genetics. Plant evolution --- Palaeobotany --- 581.6 --- 631.52 --- 903.28 --- Agriculture, Prehistoric --- Plants, Cultivated --- -#WPLT:ecol --- Cultivated plants --- Agriculture --- Domestication --- Plant introduction --- Plants, Useful --- Prehistoric agriculture --- Prehistoric peoples --- Applied botany. Use of plants. Technobotany. Economic botany --- Improvement of plant strains. Applied genetics. Selection etc. --- Prehistorie: voedingsoverblijfselen; keukenhulpmiddelen --- History --- Food --- ETH Ethnobotany & Economic botany --- 903.28 Prehistorie: voedingsoverblijfselen; keukenhulpmiddelen --- 631.52 Improvement of plant strains. Applied genetics. Selection etc. --- 581.6 Applied botany. Use of plants. Technobotany. Economic botany --- #WPLT:ecol --- Improvement of plant strains. Applied genetics. Selection etc
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Plante transgénique --- Transgenic plants --- Résistance aux maladies --- Disease resistance --- Impact sur l'environnement --- Environmental impact --- Santé publique --- public health --- Réglementation --- Regulations --- 577.3 --- 631.52 --- 66.098 --- -Plants --- Flora --- Plant kingdom --- Plantae --- Vascular plants --- Vegetable kingdom --- Vegetation --- Wildlife --- Organisms --- Botany --- GE crops (Genetically engineered crops) --- GE plants (Genetically engineered plants) --- Genetically engineered crops --- Genetically engineered plants --- Genetically modified crops --- Genetically modified plants --- GM crops (Genetically modified crops) --- GM plants (Genetically modified plants) --- Novel crops --- Transgenic crops --- Plants, Cultivated --- Transgenic organisms --- Plant genetic engineering --- Physical and physicochemical bases of life. Biophysics. Biophysical chemistry in general --- Improvement of plant strains. Applied genetics. Selection etc. --- Biological processes. Biotechnology --- Risk assessment --- Disease and pest resistance --- -Genetic aspects. --- 66.098 Biological processes. Biotechnology --- 631.52 Improvement of plant strains. Applied genetics. Selection etc. --- 577.3 Physical and physicochemical bases of life. Biophysics. Biophysical chemistry in general --- Genetic aspects. --- Plants, Genetically Modified --- Risk Assessment --- Pest Control, Biological --- Genetic Engineering --- Pest Control --- Genetic Techniques --- Risk --- Plants --- Risk Management --- Epidemiologic Measurements --- Organisms, Genetically Modified --- Probability --- Organization and Administration --- Organism Forms --- Eukaryota --- Public Health --- Investigative Techniques --- Communicable Disease Control --- Statistics as Topic --- Environment and Public Health --- Health Services Administration --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Public Health Practice --- Epidemiologic Methods --- Health Care Evaluation Mechanisms --- Health Care --- Quality of Health Care --- Health Care Quality, Access, and Evaluation --- Plant Sciences --- Agriculture --- Earth & Environmental Sciences --- Risk assessment. --- Disease and pest resistance&delete& --- Genetic aspects --- Improvement of plant strains. Applied genetics. Selection etc --- Immunogenetics --- Plant genetics
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