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Natural killer cell protocols : cellular and molecular methods
Authors: ---
ISBN: 0896036839 0585400652 9786610820368 1280820365 1592590446 Year: 2000 Publisher: Totowa (N.J.) : Humana press,

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Abstract

In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.

Begotten, not made : conceiving manhood in late antiquity
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ISBN: 0804739730 0804737061 Year: 2000 Publisher: Stanford (Calif.): Stanford university press

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Nicene trinitarian doctrine (when the Trinity became for the first time the centrepiece of doctrinal orthodoxy) is important not only for theological innovation but also for the redefinition of manhood in the fourth century. Exploring the significance of this movement for the subsequent history of Western masculine ideals, the author directly engages, combines, and disrupts divergent perspectives of historical theology, late Roman cultural history, and French feminist theory, bringing contemporary theorist Luce Irigaray into dialogue with the Patristic corpus. The book centers on performative readings of Athanasius of Alexandria, Gregory of Nyssa, and Ambrose of Milan, each of whom played a crucial role in defending Nicene trinitarian doctrine. Each also modelled a distinctive style of fourth-century masculine self-fashioning. The concluding chapter considers the sum of these three figures from an explicitly feminist theological and theoretical perspective.

Keywords

233 --- De mens. Theologische antropologie --- Masculinity --- Men (Christian theology) --- Religious aspects --- Christianity. --- History of doctrines. --- Gregory, --- Ambrose, --- Athanasius, --- Nicene Creed. --- Theological anthropology --- Masculinity (Psychology) --- Sex (Psychology) --- Men --- Religious aspects&delete& --- Christianity --- History of doctrines --- Ghirīghūriyūs, --- Grégoire, --- Gregor, --- Gregori, --- Gregorio, --- Grēgorios, --- Gregorius, --- Grigoli, --- Grigoriĭ, --- Grzegorz, --- Qiddīs Ghirīghūriyūs Usquf Nīṣṣ, --- Afanasiĭ, --- Aḟanasīĭ Velikīĭ, --- Atanasio, --- Atanazy, --- Atanazy Wielki, --- Athanase, --- Athanasios, --- Athanāsiyūs, --- Athnāsiyūs, --- Bābā Athanāsiyūs Baṭriyark al-Iskandarīyah, --- أثناسيوس، --- بابا اثناسيوس بطريرك الإسكندرية --- Atanasie cel Mare, --- Pseudo-Athanasius --- Ambrogio, --- Ambroise, --- Ambrosio, --- Ambrosius Mediolanensis, --- Ambrosius, --- Ambrosius von Mailand, --- Amvrosīĭ, --- Aurelio Ambrogio, --- Aurelius Ambrosius, --- Niceno-Constantinopolitan Creed --- Symbolum Nicaenum --- Nicaenische Glaubensbekenntnis --- Nizänisches Glaubensbekenntnis --- Constantinopolitan Creed --- Credo des Conciles de Nicée-Constantinople --- Symbolum Constantinopolitanum --- Glaubensbekenntnis von Nizäa-Konstantinopel --- Nikaian-Konstantinopolin uskontunnustus --- NK --- Σύμβολον της Πίστεως --- Symvolon tēs Pisteōs --- Icon/Symbol of the Faith --- Profession of Faith of the 318 Fathers --- Profession of Faith of the 150 Fathers --- Credo (Nicene Creed) --- Grigorije, --- Афанасий, --- Gregorius Nyssenus --- Gregor von Nyssa --- Gregorio di Nissa --- Gregorius van Nyssa --- Gregory of Nyssa --- Grégoire de Nysse --- Atanasije Aleksandrijski, --- Athanasios Alexandreias, --- Ambrogio --- Ambroise --- Ambrose --- Ambrosio --- Ambrosius Mediolanensis --- Ambrosius --- Ambrosius von Mailand --- Amvrosīĭ --- Aurelio Ambrogio --- Aurelius Ambrosius

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