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Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.

Affinity chromatography -- Laboratory manuals. --- Chromatography, Liquid --- Chromatography, Affinity --- Chromatography --- Chemistry Techniques, Analytical --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Human Anatomy & Physiology --- Animal Biochemistry --- Health & Biological Sciences --- Affinity chromatography --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- Chromatographic analysis

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“The human body is a machine whose movements are directed by the soul,” wrote René Descartes in the early seventeenth century. The intr- sic mechanisms of this machine gradually became clear through the hard work of Renaissance scientists. Leonardo da Vinci is one such scientist from this period of enlightenment. In pursuit of knowledge, Leonardo dissected the bodies of more than 30 men and women. He sawed the bones lengthwise, to see their internal structure; he sawed the skull, cut through the vertebrae, and showed the spinal cord. In the process, he took extensive notes and made carefully detailed sketches. His drawings d- ferentiated muscles that run across several joints from those muscles that act on a single joint. “Nature has made all the muscles appertaining to the motion of the toes attached to the bone of the leg and not to that of the thigh,” wrote Leonardo in 1504 next to one of his sketches of the lower extremity, “because when the knee joint is flexed, if attached to the bone of the thigh, these muscles would be bound under the knee joint and would not be able to serve the toes. The same occurs in the hand owing to the flexion of the elbow. ” Another Renaissance scholar who made fundamental contributions to the physiology of movement is Giovanni Alfonso Borelli. Born in 1604 in Naples, Borelli was a well-respected mathematician.

Biomechanics --- Movement --- Human mechanics --- Mécanique humaine --- Human mechanics. --- Human physiology. --- Life sciences. --- Medicine. --- Physiological Processes --- Musculoskeletal Physiological Processes --- Physiological Phenomena --- Musculoskeletal Physiological Phenomena --- Musculoskeletal and Neural Physiological Phenomena --- Phenomena and Processes --- Locomotion --- Human Anatomy & Physiology --- Biology --- Health & Biological Sciences --- Physiology --- Biology - General --- Biomedicine. --- Human Physiology. --- Life Sciences, general. --- Biomedicine general. --- Biomedicine, general. --- Biomechanical Phenomena. --- Physiology. --- Health Workforce --- Biosciences --- Sciences, Life --- Science --- Human biology --- Medical sciences --- Human body

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In this Journey to Microbial Worlds we present the diversity of microorganisms, from the state of fossil microbes in Archaean age rocks to the possibilities of extraterrestrial life. This volume discusses the extremophiles living in harsh environments (from our anthropocentric point) and describes them in considerable detail. Some chapters also review topics such as symbiosis, bacterial luminescence, methanogens, and petroleum-grown cells. The final chapters of this book shed new light on astrobiology and speculate on extremophiles as candidates for extraterrestrial life. All chapters are updated to the latest research level.

Biology --- Health & Biological Sciences --- Biology - General --- Extreme environments --- Adaptation (Physiology) --- Public Health --- Microbiology --- Physiological Processes --- Adaptation, Biological --- Environment and Public Health --- Physiological Phenomena --- Biological Processes --- Biological Science Disciplines --- Biological Phenomena --- Health Care --- Phenomena and Processes --- Natural Science Disciplines --- Disciplines and Occupations --- Adaptation, Physiological --- Environmental Microbiology --- Microbiology & Immunology --- Microbiology. --- Life sciences. --- Plant science. --- Botany. --- Aquatic ecology . --- Oceanography. --- Life Sciences, general. --- Plant Sciences. --- Freshwater & Marine Ecology. --- Oceanography, Physical --- Oceanology --- Physical oceanography --- Thalassography --- Earth sciences --- Marine sciences --- Ocean --- Aquatic biology --- Ecology --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Natural history --- Plants --- Biosciences --- Sciences, Life --- Science --- Microbial biology --- Microorganisms --- Floristic botany

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Nature Reviews Molecular Cell Biology is the leading monthly review journal in the field of molecular and cell biology. With its extraordinary breadth and depth of coverage, the journal provides a unique resource of information, opinion and commentary for cutting-edge molecular and cell biology research. All Reviews, Perspectives and Progress articles are carefully commissioned, written by leaders in the field, and subject to rigorous peer review — which results in timely and authoritative articles. In addition, articles are edited and the diagrams redrawn by professional art editors, which makes for highly readable, visually attractive articles that are accessible to specialists and non-specialists alike.

Molecular biology --- Cytology --- Cytologie --- Biologie moléculaire --- Periodicals. --- Périodiques --- Cytology. --- Molecular biology. --- Cells. --- Molecular Biology. --- 57 --- 577.21 --- 576 --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Cell --- Cell Biology --- Biological sciences in general --- Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- Cellular and subcellular biology. Cytology --- Periodicals --- Chemistry --- Life Sciences --- Biochemistry --- Biology --- General and Others --- Genetic Phenomena --- 576 Cellular and subcellular biology. Cytology --- 577.21 Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- Biologie moléculaire --- Périodiques --- EBSCOASP-E EJBIOMO EJMEDEC EPUB-ALPHA-N EPUB-PER-FT MDBIOCHE MDCYTOLO NPG-E --- Cell biology --- Cellular biology --- Molecular biochemistry --- Molecular biophysics --- Cells --- Cytologists --- Biophysics --- Biomolecules --- Systems biology --- Histology. --- moleculaire biologie --- Cancer research --- Life sciences --- Stem cells --- Developmental biology --- Life Sciences, general --- Cancer Research --- Developmental Biology --- Stem Cells --- Biochemistry, general --- Development (Biology) --- Growth --- Ontogeny --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- MDBIOCHE MDCYTOLO --- Molecular Biology