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In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods-largely based on fluorescence measurement-to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. In addition, there are methods to assess Ca2+ -sensitive target site activity. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin. These cutting-edge techniques will be of enormous value to all those working to understand not only cell signaling, but also the mode of action of a range of pharmacological agents.

546.41 --- 57.088 --- 577.2 --- Calcium Ca --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Molecular bases of life. Molecular biology --- Calcium -- Laboratory manuals. --- Calcium -- Research -- Methodology. --- Calcium channels -- Research -- Methodology. --- Cellular signal transduction -- Research -- Methodology. --- Electronic books. -- local. --- Calcium --- Calcium channels --- Cellular signal transduction --- Biochemical Processes --- Ion Channels --- Fluorometry --- Spectrum Analysis --- Second Messenger Systems --- Cell Physiological Processes --- Blood Coagulation Factors --- Ion Transport --- Metals, Alkaline Earth --- Biochemical Phenomena --- Membrane Transport Proteins --- Luminescent Measurements --- Biological Factors --- Biological Transport --- Chemistry Techniques, Analytical --- Metals --- Cell Physiological Phenomena --- Membrane Glycoproteins --- Elements --- Chemical Processes --- Calcium Signaling --- Spectrometry, Fluorescence --- Calcium Channels --- Signal Transduction --- Investigative Techniques --- Membrane Proteins --- Photometry --- Chemicals and Drugs --- Inorganic Chemicals --- Chemical Phenomena --- Carrier Proteins --- Metabolism --- Phenomena and Processes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Proteins --- Metabolic Phenomena --- Amino Acids, Peptides, and Proteins --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Laboratory manuals --- Research --- Methodology --- Methodology. --- Laboratory manuals. --- 577.2 Molecular bases of life. Molecular biology --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 546.41 Calcium Ca --- Alkaline earth metals --- High-calcium diet --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Channels, Calcium --- Ion channels --- Research&delete& --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- CALCIUM CHANNELS --- CALCIUM --- SPECTROMETRY, FLUORESCENCE --- MICROSCOPY, CONFOCAL --- CA(2+)-TRANSPORTING ATPase --- CALMODULIN --- METABOLISM --- ADMINISTRATION AND DOSAGE