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Fermentation --- État physique --- physical states --- Trichoderma --- Trichoderma longibrachiatum --- Aminosucre --- amino sugars --- 663.14 --- Fermentation of yeast. Baker's yeast. Pressed yeast. Nutritional yeast --- Theses --- 663.14 Fermentation of yeast. Baker's yeast. Pressed yeast. Nutritional yeast

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The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

Pichia --- Pichia pastoris --- Yeast fungi --- Genetic vectors --- Levures (Botanique) --- Vecteurs de clonage --- genetics --- Biotechnology --- Biotechnologie --- -Yeasts --- Fungi --- Basidiomycetes --- Blastomycetes --- Endomycetales --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- genetics. --- Genetic vectors. --- Pichia pastoris. --- Biotechnology. --- -genetics. --- Genetics. --- Yeast --- Yeast biotechnology --- Microbial biotechnology --- Yeast industry --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- GENETIC VECTORS --- PICHIA --- PICHIA PASTORIS --- YEAST FUNGI --- GENETICS --- BIOTECHNOLOGY

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582.282.232 --- 663.12 --- 575.113 --- 577.212 --- 57.083.1 --- Sacharomycetaceae --- Yeasts in general. Brewing yeast. Ascending and descending yeasts --- Gene. Genetic apparatus. Genome --- Central dogma of molecular biology. Coding of inheritance information. The genetic code and its chemical nature --- Microbiological methods and techniques --- 57.083.1 Microbiological methods and techniques --- 577.212 Central dogma of molecular biology. Coding of inheritance information. The genetic code and its chemical nature --- 575.113 Gene. Genetic apparatus. Genome --- 663.12 Yeasts in general. Brewing yeast. Ascending and descending yeasts --- 582.282.232 Sacharomycetaceae --- genomes --- nomenclature --- Yeasts --- chromosomes --- gene expression --- proteins --- Immunology --- Mutation --- mutation --- Nomenclature --- Yeast fungi --- Genetics --- mutation. --- nomenclature.