Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Life Sciences --- Biology

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Biology --- Life sciences --- Biological Sciences --- Periodicals --- Arts and Humanities --- Life Sciences --- History

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Over the past three decades, the philosophy of biology has emerged from the shadow of the philosophy of physics to become a respectable and thriving philosophical subdiscipline. In their book, the authors take a fresh look at the life sciences and their philosophy from a strictly realist and emergentist-naturalist perspective. They outline a unified and science-oriented philosophical framework that enables them to clarify many foundational and philosophical issues in biology. Thus, this book should be of interest to both life scientists and philosophers and is suitable as a textbook for courses at the advanced levels as well as for independent study.

Biology --- Philosophy. --- Life sciences. --- Medicine. --- Life Sciences, general. --- Biomedicine, general. --- Philosophy, general. --- Mental philosophy --- Humanities --- Health Workforce --- Biosciences --- Sciences, Life --- Science --- Vitalism --- Biology - Philosophy.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

In Helicobacter pylori Protocols, leading experts bring together an outstanding collection state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for culturing H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed to solve the difficult technical problems created by the application of today's powerful bacterial genetic and molecular cloning techniques to H. pylori. Helicobacter pylori Protocols contains a wide variety of stand-alone techniques that are unfailingly reproducible. The book is certain to become a standard experimental and clinical laboratory reference for all those engaged in H. pylori work today.

Helicobacter pylori --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer­ ing is divided into seven parts, each containing a collection of chapters address­ ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod­ ucts, depending on whether a specific restriction site can be used in the clon­ ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro­ tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom gene, as well as one chapter describing a specific use of in vitro selection. Part IV addresses the frequent need to amplify and clone segments of DNA that are to the right, left, or scattered within a stretch of DNA (either vector, chromosomal, or cDNA) of known sequence.

Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition