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Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants. Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.

Agricultural biotechnology --- Agrobacterium --- Agricultural biotechnology. --- Agrobacterium. --- Agro-biotechnology --- Biotechnology --- Polymonas --- Rhizobiaceae --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.

Histology. Cytology --- Chemistry --- Histocytochemistry --- Cytology --- Histochemistry --- Cytologie --- Histochimie --- periodicals --- Periodicals --- Périodiques --- Cell Biology. --- Histocytochemistry. --- Cytology. --- Histochemistry. --- Biochemistry --- Cellular Biology --- Biologies, Cell --- Biologies, Cellular --- Biology, Cell --- Biology, Cellular --- Cell Biologies --- Cellular Biologies --- Cytochemistry --- Histology --- Immunology --- General and Others --- Health Sciences --- Life Sciences --- Biology --- Chemistry. --- General and Others. --- Health Sciences. --- Life Sciences. --- Histology. --- Cell Biology --- periodicals. --- Périodiques --- EBSCOASP-E EJBIOMO EJMEDEC EPUB-ALPHA-H EPUB-PER-FT MDBIOCHE MDCYTOLO MDHISTOL SPRINGER-E --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Cytologie.

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The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under­ standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia­ tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio­ technological products of industrial importance.

Plant genetic engineering --- Plant gene expression --- Crops --- Laboratory manuals --- Genetic engineering --- Laboratory manuals. --- Gene expression --- Plant molecular genetics --- Agricultural crops --- Crop plants --- Farm crops --- Industrial crops --- Farm produce --- Plants, Cultivated --- Agronomy --- Crop science --- Plant products --- Plants --- Plant biotechnology --- Transgenic plants --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Plant genetic engineering - Laboratory manuals --- Plant gene expression - Laboratory manuals --- Crops - Genetic engineering - Laboratory manuals

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Gene transfer is an essential technology for improving our under­ standing of gene structure and function. Although there are many meth­ ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi­ ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in­ cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna­ tives to electric field-based methods, citing the advantages and limita­ tions of the various methods for achieving specific goals.

Plant genetic engineering --- Plant genetic transformation --- Crops --- Electroporation --- Electrofusion --- Cell Fusion --- Plants --- Genetic Techniques --- Cytological Techniques --- Cell Physiological Processes --- Clinical Laboratory Techniques --- Electrochemical Techniques --- Investigative Techniques --- Eukaryota --- Cell Physiological Phenomena --- Organisms --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Plant Ecology --- Botany --- Earth & Environmental Sciences --- Laboratory manuals --- Genetic engineering --- Laboratory manuals. --- Bioelectrochemistry --- Cytology --- Membrane fusion --- Agricultural crops --- Crop plants --- Farm crops --- Industrial crops --- Farm produce --- Plants, Cultivated --- Agronomy --- Crop science --- Plant products --- Plant transformation --- Genetic transformation --- Plant biotechnology --- Transgenic plants --- Technique --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important.

Protein Biosynthesis --- Transcription, Genetic --- Genetic Techniques --- Molecular Biology --- Molecular genetics --- Genetic transcription --- Genetic translation --- Génétique moléculaire --- Transcription génétique --- Traduction génétique --- Laboratory Manuals --- Laboratory manuals --- Manuels de laboratoire --- Moleuclar genetics --- Peptide Biosynthesis --- Biochemical Processes --- Gene Expression --- Biochemistry --- Genetics --- Investigative Techniques --- Chemical Processes --- Metabolism --- Biology --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genetic Processes --- Chemistry --- Biochemical Phenomena --- Chemical Phenomena --- Natural Science Disciplines --- Genetic Phenomena --- Metabolic Phenomena --- Disciplines and Occupations --- Phenomena and Processes --- Health & Biological Sciences --- Laboratory manuals. --- -Genetic transcription --- -Genetic translation --- -577.2 --- 57.088 --- Translation (Genetics) --- Genetic code --- Genetic regulation --- Transcription (Genetics) --- Molecular bases of life. Molecular biology --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 577.2 Molecular bases of life. Molecular biology --- 577.2 --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Cytology.