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Molecular biology --- DNA --- Conformation --- Conformation. --- Basic Sciences. Molecular Biology -- Nucleic Acids --- ALLW. --- Basic Sciences Molecular Biology -- Nucleic Acids. --- CONFORMATION --- STRUCTURE

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Understanding the mechanisms and control of gene transcription is one of the central goals for a large proportion of molecular biologists currently involved in research. This book concentrates on RNA polymerase II transcription of eukaryotic protein-coding systems but many of the approaches and techniques described apply equally well to the study of systems involving RNA polymerases I and III. This book covers all the major current procedures and approaches in this field, including not only the analysis of transcription in vitro and in vivo but also the identification, purification and characterization of transcription factors and their interaction with specific DNA target sites. Appendices list useful reference data including details of all known transcriptional control cis-elements and trans-factors for RNA polymerase II genes, complete with a full bibliography.

Gene expression --- Genetic transcription --- Gene Expression --- Gene Expression Regulation --- Transcription, Genetic --- Technique --- Research --- Methodology --- Basic Sciences. Molecular Biology --- Technique. --- Methodology. --- Genetic Engineering --- Genetic Engineering. --- Gene Expression. --- Gene Expression Regulation. --- Transcription, Genetic.

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Biotechnology --- Genetic engineering --- Biotechnologie --- Génie génétique --- Public opinion --- Moral and ethical aspects --- Surveys --- Opinion publique --- Aspect moral --- Enquêtes --- -Genetic engineering --- -Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Transgenic organisms --- Chemical engineering --- Basic Sciences. Molecular Biology --- Public opinion. --- Genetic Engineering --- -Public opinion --- Genetic Engineering. --- Génie génétique --- Enquêtes --- Designed genetic change --- Biotechnology - European Economic Community countries --- Genetic engineering - European Economic Community countries - Public opinion