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Over the past twenty years, the disciplines of cellular and molecular biology have generated a bounty of information pertaining to the understanding of the structure and function of eukaryotic cells . It is now recognized that individual cells regulate their activities in response to a multitude of external signals ; that external signals, a- ing through a limited number of second messenger systems, modulate the cell's pattern of gene expression; and that the cohort of genes available for regulation is dependent upon the developmental history of the cell. Extrapolating these truths to the whole organism is a challenge that has been met by an increasing number of investigators . The wealth of information that molecular and cell biology has provided us with is now being integrated into an understanding of whole t- sues, organs, and organisms. Without such an integration, the molecular information will be nothing more than a confusing catalog of sequences and structures. Central to this project will be the app- cation of the powerful new techniques described in Transgenesis Techniques: Principles and Protocols .

Animals, Transgenic --- Genetic Engineering --- Transformation, Genetic --- Animal genetic engineering --- Genetic transformation --- Transgenic animals --- Animaux transgéniques --- methods --- veterinary --- Technique --- -Genetic transformation --- -Transgenic animals --- Animals --- Transgenic organisms --- Gene transfer --- Transformation (Genetics) --- Genetic recombination --- Microbial genetics --- Nucleic acids --- Transfection --- Animal biotechnology --- Genetic engineering --- Transgenic animals. --- Technique. --- -575.08:577.21 --- 636.082.4 --- 59.084 --- Genetic engineering, genetic manipulation, recombinant DNA --- Breeding methods and techniques. Reproduction --- Laboratory animals --- 59.084 Laboratory animals --- 636.082.4 Breeding methods and techniques. Reproduction --- 575.08:577.21 Genetic engineering, genetic manipulation, recombinant DNA --- Animals, Genetically Modified. --- Animaux transgéniques --- Transformation, Genetic. --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- 575.08:577.21 --- methods. --- veterinary. --- Cytology.

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