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The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

Biological transport --- Biological Transport --- Cell Membrane Permeability --- Permeability, Cell Membrane --- Ionophores --- Biologic Transport --- Transport, Biological --- Transport, Biologic --- Transport Vesicles --- Membrane Transport Proteins --- Membrane transport --- Passive transport, Biological --- Physiological transport --- Diffusion --- Osmosis --- Energy Metabolism. --- Membranes (Biology) --- Biomembranes --- General biochemistry --- Biological transport. --- Bioenergetics. --- Energy metabolism. --- Active oxygen. --- Antioxidants. --- Bacterial cell walls. --- Bacterial proteins. --- Biochemistry. --- Biological psychiatry. --- Biological systems. --- Brain --- Cell Membrane --- Cell Membrane. --- Chromatin --- Chromatin. --- Complexity (Philosophy). --- Disease --- Free Radicals. --- Free radicals (Chemistry) --- Gene Expression --- Gene expression. --- Genetic Engineering --- Genetic Vectors. --- Genetic engineering. --- Genetic transformation. --- Glycoproteins --- Glycoproteins. --- Life (Biology). --- Lipid membranes. --- Lipids --- Lipids. --- Lipoproteins --- Lipoproteins. --- Membrane Lipids --- Membrane Proteins --- Membrane lipids --- Membrane lipids. --- Membrane proteins. --- Membranes --- Mental disorders --- Neurotransmitter receptors. --- Peptidoglycans. --- Principal components analysis. --- Protein binding. --- Reactive Oxygen Species. --- Reduction (Chemistry). --- Transformation, Genetic --- Viruses. --- Pathophysiology. --- Physiology. --- Metabolism. --- Ultrastructure. --- Etiology. --- Methods. --- Metabolism --- Disorders. --- Anatomy and histology. --- Bioenergetics --- Energy metabolism --- Energy Metabolism --- Biochemistry --- Microbial respiration --- Energy balance (Biology) --- Energy budget (Biology) --- Energy dynamics (Ecology) --- Energy utilization (Biology) --- Energy budget (Geophysics) --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- anatomy & histology --- Composition --- 547.96 --- 547.96 Proteins --- Proteins --- 577.23 --- 577.23 Energy bases of life. Molecular bioenergetics --- Energy bases of life. Molecular bioenergetics --- Permeability