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Biochemie --- Biochemistry --- Biochimie --- Biological chemistry --- Chemistry [Biological ] --- Chemistry [Physiological ] --- Biochemistry.

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General biochemistry --- Molecular biology --- Biochemie --- Biochemistry --- Biochimie --- Biological chemistry --- Biologie [Moleculaire ] --- Biologie moléculaire --- Biology [Molecular ] --- Chemistry [Biological ] --- Chemistry [Physiological ] --- Moleculaire biologie --- Molecular biochemistry --- Molecular biophysics --- Biochemistry. --- Molecular biology.

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DNA --- Pulsed-field gel electrophoresis --- Electrophoresis, Gel, Pulsed-Field --- Genetic Techniques. --- Analysis. --- Technique. --- methods. --- Pulsed-field gradient electrophoresis --- Gel electrophoresis --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Key Features * Matrix activation * Ligand immobilization * Binding and elution of target molecules * Enzyme catalysis on solid supports * Analytical affinity chromatography * Isolation/purification of antibodies * Preparation of antibody fragments * Immunoaffinity chromatography * Immobilization of nucleic acids * Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: * Matrices * Spacers * Methods of activation and coupling Background information and insights on: * Affinity interactions * The ease and power of affinity chromatography * Attaching molecules to insoluble supports * Matrices currently in use * Over 20 methods of activation * Spacers * Extensive References

Chromatography, Affinity. --- Ligands. --- Protein Binding. --- Affinity chromatography --- Immobilized ligands (Biochemistry) --- Immunoadsorption --- Ligand binding (Biochemistry) --- Binding, Ligand (Biochemistry) --- Biochemistry --- Dye-ligand affinity chromatography --- Radioligand assay --- Adsorption (Biology) --- Antigen-antibody reactions --- Immunochemistry --- Ligands (Biochemistry) --- Chromatographic analysis --- Affinity Chromatography --- Bioaffinity Chromatography --- Chromatography, Bioaffinity --- Affinity chromatography. --- Immunoadsorption. --- Basic Sciences. Biology --- Biological Techniques --- Immobilized ligands (Biochemistry). --- Ligand binding (Biochemistry). --- Biological Techniques. --- Chromatography, Affinity --- Ligands --- Protein Binding --- Immunochromatography --- BIOCHEMISTRY --- immunoadsorption --- ligand binding --- immobilized ligands --- Monograph --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Ligand --- Plasma Protein Binding Capacity --- Binding, Protein --- Binding Sites --- Blood Proteins --- Protein Interaction Mapping

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Affinity chromatography. --- Proteins --- Analysis. --- Affinity chromatography --- Chromatography, Affinity --- Chromatography, Liquid --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Chromatography --- Chemistry Techniques, Analytical --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Analysis --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Chromatographies --- Liquid Chromatography --- Affinity Chromatography --- Bioaffinity Chromatography --- Chromatography, Bioaffinity --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition