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CELL BIOLOGY --- CHROMOSOMES --- STAINING --- LEUKOCYTES --- CHEMOTAXIS --- NUCLEOLUS --- ISOLATION --- MITOTIC APPARATUS --- CELL NUCLEUS --- METAPHASE ARREST --- MUTANTS --- MAMMALS --- CELLS --- REPLICA PLATING --- CELL CYCLE --- ISOLEUCINE --- G1 ARREST --- GRADIENT CENTRIFUGATION --- SYNCHRONIZATION --- SYNTHETIC MEDIA --- CELL CULTURE --- METHODS