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With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.

Environmental sciences. --- Inclusion Bodies --- Escherichia coli --- Filamentous fungi --- Microalgae --- Recombinant Proteins --- Microorganism --- fusion tags --- yeast

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"Animal Biotelemetry is an open access peer-reviewed journal that publishes the results of studies utilizing telemetric techniques (including biologgers) to understand physiological, behavioural, and ecological mechanisms in a broad range of environments (e.g. terrestrial, freshwater and marine) and taxa. The journal also welcomes descriptions and validations of newly developed tagging techniques and tracking technologies, as well as methods for analyzing telemetric data."

Animal radio tracking --- Biotelemetry --- Radiopistage --- Biotélémétrie --- Periodicals --- Periodicals. --- Périodiques --- Animal radio tracking. --- Biotelemetry. --- Biological telemetry --- Telemetry, Biological --- Telemetry in biological research --- Animal radio tagging --- Animals --- Radio tagging, Animal --- Radio tracking, Animal --- Tagging, Animal radio --- Tagging, Wildlife radio --- Tracking, Animal radio --- Tracking, Wildlife radio --- Wildlife biotelemetry --- Wildlife radio tagging --- Wildlife radio telemetry --- Wildlife radio tracking --- Wildlife telemetry --- Bio-logging (Animal radio tracking) --- Biologging (Animal radio tracking) --- Radio tracking --- ecology --- tracking systems --- electronic tags --- animal physiology --- animal behaviour --- telemetry --- Bioengineering --- Electronic measurements --- Radio telemetry --- Tracking and trailing --- Wildlife research --- Zoology --- Technique --- General biochemistry --- General ecology and biosociology

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Pastoral care. --- Race relations. --- General Conference of Seventh-Day Adventists. --- Integration, Racial --- Race problems --- Race question --- Relations, Race --- Ethnology --- Social problems --- Sociology --- Ethnic relations --- Minorities --- Racism --- Care of souls --- Cure of souls --- Church work --- Pastoral counseling --- Pastoral theology --- Seventh-Day Adventists (Group) --- Che Chʻiril Ansigil Yesu Chaerim Kyohoe --- Che 7-il Ansigil Yesu Chaerim Kyohoe --- Chaerim Kyohoe --- SDA --- Adventisti sedmog dana --- Adventysty sʹomoho dni︠a︡ --- Subotnyky --- Gemeinschaft der Siebenten-Tags-Adventisten --- S.D.A. --- Igreja Adventista do Sétimo Dia --- IASD --- I.A.S.D. --- H. N. Adventista Egyház --- Hetedik Napos Adventista Egyház --- Hetednapos Adventista Egyház --- Adventista Egyház --- Sjundedags adventisterna --- T︠S︡erkovʹ khristian adventistov sedʹmogo dni︠a︡ --- Iglesia Adventista del Séptimo Día --- Adventisti ot sedmii︠a︡ den --- Seventh-Day Adventist Church --- Sewendedag-Adventistekerk --- Chiesa avventista del 7o giorno --- Syvende dags adventistkirken --- Keresztény Advent Közösség

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Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research. Proteomics offers one of the best options for the functional analysis of translated regions of the genome, generating a wealth of detailed information regarding the intrinsic mechanisms of plant stress responses. Various proteomic approaches are being exploited extensively for elucidating master regulator proteins which play key roles in stress perception and signaling, and these approaches largely involve gel-based and gel-free techniques, including both label-based and label-free protein quantification. Furthermore, post-translational modifications, subcellular localization, and protein–protein interactions provide deeper insight into protein molecular function. Their diverse applications contribute to the revelation of new insights into plant molecular responses to various biotic and abiotic stressors.

14-3-3 proteins --- n/a --- targeted two-dimensional electrophoresis --- somatic embryogenesis --- nitrogen metabolism --- subtilase --- Sporisorium scitamineum --- non-orthodox seed --- antioxidant activity --- sweet potato plants infected by SPFMV --- photosynthesis --- B. acuminata petals --- chlorophyll deficiency --- seed proteomics --- imbibition --- pollination --- Sarpo Mira --- qRT-PCR --- holm oak --- tuber phosphoproteome --- isobaric tags for relative and absolute quantitation (iTRAQ) --- Quercus ilex --- nucleotide pyrophosphatase/phosphodiesterase --- lettuce --- ?-subunit --- protein phosphatase --- germination --- drought stress --- pyruvate biosynthesis --- weakening of carbon metabolism --- differential proteins --- heterotrimeric G protein --- organ --- LC-MS-based proteomics --- potato proteomics --- smut --- gel-free/label-free proteomics --- ? subunit --- shotgun proteomics --- 2D --- chloroplast --- proteome functional annotation --- Phalaenopsis --- Clematis terniflora DC. --- wheat --- Dn1-1 --- carbon metabolism --- physiological responses --- Zea mays --- phenylpropanoid biosynthesis --- ISR --- mass spectrometric analysis --- patatin --- leaf --- pea (Pisum sativum L.) --- maize --- ergosterol --- Camellia sinensis --- seed storage proteins --- silver nanoparticles --- elevated CO2 --- metacaspase --- SPV2 and SPVG --- SnRK1 --- MALDI-TOF/TOF --- (phospho)-proteomics --- leaf spot --- rice isogenic line --- wheat leaf rust --- pathway analysis --- phosphoproteome --- sugarcane --- senescence --- Oryza sativa L. --- Arabidopsis thaliana --- heat stress --- gene ontology --- innate immunity --- Pseudomonas syringae --- bolting --- chlorophylls --- shoot --- Simmondsia chinensis --- RT-qPCR --- stresses responses --- Solanum tuberosum --- seeds --- GC-TOF-MS --- sucrose --- proteome --- Puccinia recondita --- cultivar --- Zea mays L. --- secondary metabolism --- ROS --- Ricinus communis L. --- after-ripening --- cadmium --- Stagonospora nodorum --- virus induced gene silencing --- quantitative proteomics --- sweet potato plants non-infected by SPFMV --- affinity chromatography --- population variability --- GS3 --- fungal perception --- ammonium --- transcriptome profiling --- mass spectrometry analysis --- papain-like cysteine protease (PLCP) --- cold stress --- nitrate --- late blight disease --- early and late disease stages --- seed imbibition --- lesion mimic mutant --- protease --- proteome map --- seed dormancy --- petal --- 2-DE proteomics --- 2D DIGE --- root --- Phytophthora infestans --- differentially abundant proteins (DAPs) --- polyphenol oxidase --- degradome --- flavonoid --- 14-3-3 --- caspase-like --- proteomics --- RGG4 --- co-infection --- plasma membrane --- chlorotic mutation --- Medicago sativa --- RGG3 --- glycolysis --- barley --- 2-DE --- protein phosphorylation --- western blotting --- N utilization efficiency --- rice --- plant pathogenesis responses --- high temperature --- data-independent acquisition --- pattern recognition receptors --- vegetative storage proteins --- leaf cell wall proteome --- plant-derived smoke --- iTRAQ --- starch --- proteome profiling --- Morus

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Integrative omics of plants in response to stress conditions play more crucial roles in the post-genomic era. High-quality genomic data provide more deeper understanding of how plants to survive under environmental stresses. This book is focused on concluding the recent progress in the Protein and Proteome Atlas in plants under different stresses. It covers various aspects of plant protein ranging from agricultural proteomics, structure and function of proteins, and approaches for protein identification and quantification.

phosphoproteomics --- GLU1 --- somatic embryogenesis --- CHA-SQ-1 --- nitrogen fertilizer --- chilling stress --- differentially abundant proteins --- ATP synthase --- photosynthetic parameters --- photosynthesis --- constitutive splicing --- phosphorylation --- Jatropha curcas --- plants under stress --- postharvest freshness --- Alternanthera philoxeroides --- rubber latex --- Millettia pinnata --- molecular and biochemical basis --- filling kernel --- drought stress --- comparative proteomic analysis --- domain --- micro-exons --- phylogeny --- phos-tagTM --- E. angustifolia --- root cell elongation --- ABA --- pollen abortion --- lncRNA --- transcriptome --- radish --- redox homeostasis --- Nelumbo nucifera --- sugar beet --- shotgun proteomics --- proteomes --- high-temperature stress --- post-genomics era --- model plant --- salt tolerance --- miRNA --- wheat --- physiological response --- stress --- visual proteome map --- transcriptional dynamics --- leaf --- maize --- Dunaliella salina --- phosphatidylinositol --- S-adenosylmethionine decarboxylase --- Gossypium hirsutum --- flavonoid biosynthesis --- phosphatase --- wood vinegar --- heat shock proteins --- silicate limitation --- purine metabolism --- natural rubber biosynthesis --- ancient genes --- cotton --- rubber grass --- abiotic stress --- heat stress --- maturation --- low-temperature stress --- molecular basis --- transcriptome sequencing --- ROS scavenging --- widely targeted metabolomics --- transdifferentiation --- seed development --- alternative splicing --- cultivars --- inositol --- salt stress --- chlorophyll fluorescence parameters --- proteome --- carbon fixation --- AGPase --- transcript-metabolite network --- molecular mechanisms --- Triticum aestivum L. --- Zea mays L. --- ROS --- label-free quantification --- woody oilseed plants --- heat-sensitive spinach variety --- MIPS --- quantitative proteomics --- regulated mechanism --- two-dimensional gel electrophoresis --- potassium --- glutathione --- Salinity stress --- integrated omics --- diatom --- ATP synthase CF1 alpha subunit (chloroplast) --- root --- proteome atlas --- brittle-2 --- mass spectrometry --- genomics --- Taraxacum kok-saghyz --- cytomorphology --- proteomics --- arbuscular mycorrhizal fungi --- signaling pathway --- proteomic --- loss-of-function mutant --- rice --- seedling --- wucai --- leaf sheath --- root and shoot --- antioxidant enzyme --- exon-intron structure diversity --- isobaric tags for relative and absolute quantitation --- regulation and metabolism --- concerted network --- drought --- heat response --- VIGS --- iTRAQ --- nitrogen use efficiency (NUE) --- stem