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De PCR-techniek heeft een revolutie in de moleculaire biologie ontketend en heeft tegenwoordig in verschillende laboratoria in uiteenlopende vakgebieden een vaste plaats ingenomen. Dit toegankelijke en rijk geïllustreerde boek vat alles samen wat je over de moderne PCR-praktijk moet weten. 

Niet alleen de fasen van de PCR-reactie, de functie van de PCR-componenten en de ontwikkeling van de PCR-primers, maar ook de nucleïnezuur-isolatie en de analyse van PCR-producten worden besproken. Daarnaast komen de real-time PCR en enkele basisbegrippen van kwaliteitszorg op het PCR-laboratorium aan de orde. Dit boek is een ideaal uitgangspunt voor studenten en laboratoriummedewerkers met geen of beperkte voorkennis van de PCR.

DNA --- PCR-Techniek --- Macromolecules --- Chemical laboratory practice --- Molecular biology --- PCR (polymerase chain reaction) --- laboratoria chemie --- DNA (deoxyribonucleic acid) --- polymeren --- moleculaire biologie --- 57.088 --- PCR --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- PCR-techniek

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Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments.A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for e

Biological techniques --- Histology. Cytology --- Cell separation --- Cellules --- Séparation --- Cell Separation --- methods --- 57.088 --- 576.3 --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- 576.3 General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Cell separation. --- Molecular biology --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Technique. --- Separation --- Cell Separation - methods

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Molecular biology --- Analytical biochemistry --- Clone Cells --- Cloning, Molecular --- Genetic Engineering --- Genetics, Microbial --- Recombination, Genetic --- Eukaryotic cells --- Molecular cloning --- Clonage moléculaire --- laboratory manuals --- Laboratory manuals --- Manuels de laboratoire --- -Cloning, Molecular --- molecular cloning --- 57.088 --- Analyse (klinische biologie) --- Bacteriofagen --- Bacteriologie --- Chemie --- Enzymen --- Genetica (erfelijkheidsleer) --- Klonen --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Cloning, Molecular. --- -57.082.261 --- -#WSCH:WBIO --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Eucaryotic cells --- Cells --- Protista --- Molecular Cloning --- Cosmids --- Cloning, Organism --- Biologische wetenschappen in het algemeen. Biologie--?.082.261 --- Moleculair klonen --- 577.2 --- klonen --- moleculaire biologie --- colibacil --- proteine isolatie --- oligonucleotide --- dna --- polymerase --- mutagenese --- proteinezuivering --- gelelektroforese --- rna --- enzyme --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Clonage moléculaire --- Clone Cells. --- laboratory manuals. --- #WSCH:WBIO --- 57.082.261 --- -molecular cloning --- Molecular cloning. --- Eukaryotic cells. --- Laboratory manuals. --- Laboratories. --- Laboratory facilities --- Research buildings --- Science laboratories --- Scientific laboratories --- Science rooms and equipment --- Monograph --- Eukaryotic cells - - Laboratory manuals --- Cloning, Molecular - laboratory manuals --- CLONING, MOLECULAR --- MOLECULAR CLONING --- PLASMIDS --- VIRUS, BACTERIAL --- DNA --- RNA --- AMINO ACID SEQUENCE --- EUKARYOTIC CELLS --- LABORATORY MANUALS --- ENZYMES --- GEL ELECTROPHORESIS --- MUTAGENS --- GENETIC VECTORS --- BACTERIOPHAGE LAMBDA --- COSMIDS --- ELECTROPHORESIS --- RNA, MESSENGER --- GENE LIBRARY --- NUCLEIC ACID PROBES --- OLIGONUCLEOTIDE PROBES --- SEQUENCE ANALYSIS, DNA --- GENE AMPLIFICATION --- MUTAGENESIS --- CELLS, CULTURED --- ESCHERICHIA COLI --- ANTIBODIES --- METHODS --- GENETICS

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After spending over 12 years developing new microsystems for biotechnology û especially concerned with the microfluidic aspects of these devices û Jean Berthier is considered a leading authority in the field. Now, following the success of his book, Microfluidics for Biotechnology, Dr. Berthier returns to explain how new miniaturization techniques have dramatically expanded the area of microfluidic applications and microsystems into microdrops and digital microfluidics.Engineers interested in designing more versatile microsystems and students who seek to learn the fundamentals of micro

Atomizers --- Drops --- Electrostatic atomization --- Microdroplets --- Microelectromechanical systems --- Microfluidics --- Spraying equipment --- 532.64 --- 544.722.3 --- 57.088 --- 631.347.3 --- 66.063.5 --- 66.069.832 --- Spraying --- Agricultural implements --- Agricultural machinery --- Gardening --- Fluidics --- Nanofluids --- MEMS (Microelectromechanical systems) --- Micro-electro-mechanical systems --- Micro-machinery --- Microelectromechanical devices --- Micromachinery --- Micromachines --- Micromechanical devices --- Micromechanical systems --- Electromechanical devices --- Microtechnology --- Mechatronics --- Atomization --- Drips --- Droplets --- Liquids --- Spheroidal state --- Nebulizers --- Perfumizers --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 532.64 Wetting angle. Contact angle --- Wetting angle. Contact angle --- 66.063.5 Wetting. Moistening. Wetting agents --- Wetting. Moistening. Wetting agents --- 631.347.3 Spraying equipment. Sprinklers. Sprays, spray-guns, jets --- Spraying equipment. Sprinklers. Sprays, spray-guns, jets --- Methodology --- Equipment and supplies --- Wettability. Chemical nature of wetting. Spreading. Spreading ability --- Atomizing. Spraying. Atomizers and spraying devices --- Equipment --- Engineering --- Mechanical Engineering --- Atomizers. --- Microelectromechanical systems. --- Equipment and supplies. --- Methodology. --- Coating processes