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Polymerase chain reaction --- Methodology. --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases

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PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary PCR diagnosticians include such considerations in their work. Extensive experience with PCR technology in both research and diagnostic applications enables researchers to pinpoint these practical limitations, and therefore instruct the user in approaches that avoid these common errors.This E-book discusses the basic concepts, chemistries, and in

Polymerase chain reaction --- Polymerase chain reaction. --- DNA probes. --- Microorganisms --- Deoxyribonucleic acid probes --- Gene probes --- Genomic probes --- Probes, DNA --- Deoxyribonucleotides --- Nucleic acid probes --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Molecular diagnosis --- Diagnostic use.

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Since the enormously successful first edition of Forensic DNA Typing was published, the Human Genome Project has published a draft sequence of the human genome and completed the "finished? reference sequence. The advent of modern DNA technology has resulted in the increased ability to perform human identity testing-desirable in a number of situations including the determination of perpetrators of violent crime such as murder and rape, resolving unestablished paternity, and identifying remains of missing persons or victims of mass disasters. The technology has been utilized in identifying remai

DNA fingerprinting. --- DNA --- Forensic genetics. --- Polymerase chain reaction. --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Forensic biology --- Genetics --- Medical jurisprudence --- Deoxyribonucleic acid synthesis --- Deoxyribonucleic acid --- Desoxyribonucleic acid --- Thymonucleic acid --- TNA (Nucleic acid) --- Deoxyribose --- Nucleic acids --- Genes --- DNA fingerprints --- DNA identification --- DNA profiles --- DNA profiling --- Genetic fingerprinting --- Genetic fingerprints --- Forensic genetics --- Identification --- Physiology. --- Synthesis. --- Technique

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This book offers a state-of-the-art overview on non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. The importance of non-amplified DNA sequencing technologies will be also discussed. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in the field.

DNA Viruses. --- DNA. --- DNA --- Polymerase chain reaction --- Genomics --- Analytical biochemistry --- Biology --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Biophysics --- Analysis --- Polymerase chain reaction. --- Genomics. --- Analytical biochemistry. --- Analysis. --- Analytic biochemistry --- Genome research --- Genomes --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Research --- Physics. --- Analytical chemistry. --- Biophysics. --- Biological physics. --- Biomedical engineering. --- Biophysics and Biological Physics. --- Analytical Chemistry. --- Biomedical Engineering. --- Biochemistry --- Chemistry, Analytic --- Molecular genetics --- Polymerization --- DNA polymerases --- Biological and Medical Physics, Biophysics. --- Biomedical Engineering and Bioengineering. --- Clinical engineering --- Medical engineering --- Bioengineering --- Engineering --- Medicine --- Analysis, Chemical --- Analytical chemistry --- Chemical analysis --- Metallurgical analysis --- Mineralogy, Determinative --- Biological physics --- Medical sciences --- Physics --- Analytic chemistry --- Chemistry

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Genomes --- Gene mapping --- Nucleotide sequence --- Genetic Techniques. --- Genetics. --- Genome. --- Génomes --- Cartes chromosomiques --- Génome humain --- Réaction en chaîne de la polymérase --- Génétique humaine --- Génétique --- Gene mapping. --- Genomes. --- Nucleotide sequence. --- Séquençage --- Aspect moral --- Technique --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Chromosome mapping --- Genetic mapping --- Genome mapping --- Linkage mapping (Genetics) --- Mapping, Gene --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- methods --- Genes --- Proteome --- Nucleic acids --- Nucleotides --- Sequence alignment (Bioinformatics) --- Genetic Structures --- Genetic Phenomena --- Genomics --- Haploidy --- Genetic Counseling --- Analysis --- Polymerase chain reaction --- Human genetics --- Genetic Techniques --- Genome --- Génomes. --- Moral and ethical aspects --- Technique. --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Heredity, Human --- Human biology --- Physical anthropology --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Polymerase chain reaction. --- Moral and ethical aspects. --- Internet access --- Génomes --- Génome humain --- Réaction en chaîne de la polymérase --- Génétique humaine --- Génétique --- Génomes. --- Séquençage