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Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods

Cancer --- Immunohistochemistry. --- In situ hybridization. --- Fluorescence in situ hybridization. --- Immunodiagnosis. --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Fluorescence microscopy --- In situ hybridization --- Nucleic acid hybridization --- Immunohistology --- Histochemistry --- Immunochemistry --- Diagnosis --- Immunological aspects

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In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.

In situ hybridization --- Laboratory manuals. --- HYBRIDIZATION --- SCIENCE --- Hybridization --- Science

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Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer.This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step

Pathology, Molecular. --- Prostate --- Rectum --- Immunochemistry. --- In situ hybridization. --- Cancer. --- Diseases. --- Nucleic acid hybridization --- Biochemistry --- Immunity --- Proctology --- Molecular pathology --- Molecular biology --- Physiology, Pathological

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In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical

Immunohistochemistry. --- Medicine. --- Pathology. --- Immunohistochemistry --- Pathology, Molecular --- Immunochemistry --- Genetic Techniques --- Histocytochemistry --- Nucleic Acid Hybridization --- Immunologic Tests --- Staining and Labeling --- Histocytological Preparation Techniques --- Immunologic Techniques --- Biochemistry --- Histology --- Laboratory Techniques and Procedures --- Histological Techniques --- Investigative Techniques --- Cytological Techniques --- Clinical Laboratory Techniques --- Diagnosis --- Chemistry --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Anatomy --- Natural Science Disciplines --- Disciplines and Occupations --- In Situ Hybridization --- Gene Expression Profiling --- Biology --- Medicine --- Health & Biological Sciences --- Pathology --- Microbiology & Immunology --- Technique --- Pathology, Molecular. --- In situ hybridization. --- Nucleic acid hybridization --- Molecular pathology --- Molecular biology --- Physiology, Pathological --- Technique.

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In the past three decades, a stream of criminological inquiry has emerged which explores, measures, and theorizes crimes and harms to the environment at the micro-, mezzo-, and macro-levels. This “green criminology”, as it has come to be known, has widened the criminological gaze to consider crimes and harms committed against air, land (from forests to wetlands), nonhuman animals, and water in local, regional, national, and international areas or arenas. Accordingly, green criminology has endeavored to understand the causes and consequences of air and water pollution, biodiversity loss, climate change, corporate environmental crime (e.g., illegal waste disposal), food production and distribution, resource extraction and exploitation, and wildlife trade and trafficking, while also exploring potential responses to these issues. This book seeks to introduce the green criminological perspective to a broader social science audience. Recognizing that green criminology is not the first social science to explore the phenomena and harms at the intersections of humanity and ecology, this book offers an introduction to some of the unique insights developed over nearly 30 years of green criminological thought and scholarship to students, professors, researchers, and practitioners working in the fields of anthropology, economics, environmental humanities, environmental sociology, geography, history, and political ecology. This book contains contributions from researchers in green criminology from around the world, including early- and mid-career scholars, as well as more established voices in the field—all of whom are dedicated to exposing, understanding, and ultimately hoping to thwart further environmental degradation and despoliation.

biogeography --- ciliates --- Paramecium quindecaurelia --- cytochrome C oxidase subunit I gene --- sibling species --- species concept in protists --- bacterial symbionts --- symbiosis --- intranuclear bacteria --- Holospora --- Gortzia --- Paramecium --- Micractinium tetrahymenae --- Tetrahymena --- Utricularia --- facultative endosymbiosis --- ciliate-algae symbiosis --- Chlorella variabilis --- Micractinium conductrix --- diagnostic PCR --- ciliate–algae symbiosis --- Holospora-like bacteria --- host–parasite interactions --- 16S rRNA gene --- full-cycle rRNA approach --- TEM --- fluorescence in situ hybridization --- algal-ciliate symbiosis --- mycosporine-like amino acids --- Pelagodileptus trachelioides --- planktonic freshwater ciliates --- Stokesia vernalis --- Vorticella chlorellata --- Chlorella --- endosymbiosis --- intracellular algae --- Micractinium --- photobiont --- infection --- syngen --- n/a --- host-parasite interactions