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Diffractive lens arrays are proposed in this work for application in reflected-light confocal microscopes. They have overcome the limitations between fields of view and resolution of traditional objectives. Experiments of multi-spot confocal imaging in surface metrology and fluorescence microscopy have been demonstrated based on the proposed concepts, which have shown capabilities of high-resolution measurement over a large area.

Confocal microscopy. --- Fluorescence microscopy.

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Confocal microscopy. --- Eye --- Ophthalmology. --- Diseases --- Diagnosis. --- Medicine --- Microscopy

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Confocal microscopy. --- Dermatology. --- Medicine --- Skin --- Microscopy --- Diseases --- Microscòpia confocal --- Dermatologia --- Diagnòstic per la imatge

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Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.

Confocal microscopy. --- Fluorescence microscopy. --- Image processing -- Digital techniques. --- Microbiology -- Technique. --- Microscopy, Confocal. --- Microscopy, Electron, Scanning. --- Microscopy. --- Confocal microscopy --- Biology --- Health & Biological Sciences --- Microscopy --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Life sciences. --- Biotechnology. --- Cell biology. --- Life Sciences. --- Biological Microscopy. --- Cell Biology. --- Optics --- Cytology. --- Chemical engineering --- Genetic engineering --- Cell biology --- Cellular biology --- Cells --- Cytologists

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Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.

Biological microscopy --- Confocal microscopy. --- Microscopie confocale --- Confocal microscopy --- Microscopy --- Diagnostic Imaging --- Image Processing, Computer-Assisted --- Diagnostic Techniques and Procedures --- Computing Methodologies --- Investigative Techniques --- Imaging, Three-Dimensional --- Microscopy, Confocal --- Information Science --- Diagnosis --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemistry --- Biology --- Health & Biological Sciences --- Physical Sciences & Mathematics --- Biology - General --- Biochemistry --- EPUB-LIV-FT LIVBIOLO LIVBIOMO LIVMEDEC SPRINGER-B --- Life sciences. --- Biotechnology. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Chemical engineering --- Genetic engineering --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition