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This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field. .
Gene therapy. --- Gene Therapy. --- Therapy, Gene --- Genetic engineering --- Therapeutics
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Gene silencing --- RNA --- Gene Silencing. --- RNA Interference. --- Gene silencing. --- RNA.
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Gene Expression --- Central Nervous System --- Central nervous system --- Gene expression --- Gene Expression. --- Gene expression. --- Genes --- Expression, Gene --- Expressions, Gene --- Gene Expressions --- Expression --- Genetic regulation --- Gene Expression Profiling
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Gene silencing --- RNA --- Gene Silencing. --- RNA Interference.
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Gene amplification --- -Amplification, Gene --- Gene expression --- Polymerase chain reaction --- Congresses --- Congresses. --- -Congresses --- Amplification, Gene
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This volume provides new approaches and technologies into roles of poly(A) metabolism in translation, RNA stability, and quality control of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Deadenylation: Methods and Protocols aims to pave the way for future investigations of the complex regulatory networks that control mRNA stability and expression.
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